Journal of the Korean Pediatric Society 2002;45(7):884-890.
Published online July 15, 2002.
The Effect of Angiotensin Converting Enzyme Gene Polymorphism in Children with Henoch-Schonlein Purpura Nephritis
Chang Woo Ha1, Ji Young Kim1, Jeong Nyeo Lee2, Jeong Hwa Lee3, Woo Yeong Chung1
1Departments of Pediatrics, College of Medicine, Inje University, Busan, Korea
2Departments of Clinical Laboratory Medicine, College of Medicine, Inje University, Busan, Korea
3Departments of Molecular Biology, Busan National University, Busan, Korea
Henoch-Schonlein Purpura 신염에서 안지오텐신 전환효소 유전자 다형성의 영향
하창우1, 김지영1, 이정녀2, 이정화3, 정우영1
1인제대학교 의과대학 소아과학교실
2인제대학교 의과대학 임상병리학교실
3부산대학교 분자생물학과
Correspondence: 
Woo Yeong Chung, Email: chungwy@chollian.net
Abstract
Purpose
: Henoch-Schonlein purpura(HSP) nephritis has been reported to vary from 25 to 50% among HSP patients and is a common cause of chronic glomerulonephritis in children. In our study, we evaluated the distribution and the association of the Insertion/Deletion(I/D) polymorphism of angiotensin converting enzyme(ACE) gene with clinical manifestations, particularly proteinuria in children with HSP nephritis, compared with that in HSP.
Methods
: ACE gene polymorphism was determined in children with HSP nephritis(n=33) and HSP(n=28) who were diagnosed in Busan Paik hospital from January 1996 to June 2001. The I/D polymorphism of ACE gene was determined by PCR amplication of genomic DNA.
Results
: The ACE I/D genotype frequency was DD : 25%, ID : 50%, II : 25% in HSP and DD : 24 %, ID : 46%, II : 30% in HSP nephritis, there was no significant difference in the genotype and allele frequencies between two groups. When statistical analysis was done according to the presence of D allele, the amount of 24-hour urinary protein excretion and the incidence of moderate to heavy proteinuria(>500 mg/m2/day) at onset and last follow-up were higher in DD/ID genotype than in those in II genotype, but these differences were not statistically significant.
Conclusion
: We suggest a lack of association between I/D polymorphism of ACE gene and clinical manifestations in children with HSP nephritis. However, further follow-up studies based on a sufficient number of patients and long term follow up periods are necessary to confirm the role of I/D polymorphism of ACE gene in children with HSP nephritis.
Key Words: Insertion/deletion polymorphism, Angiotensin converting enzyme gene, Henoch-Schonlein purpura nephritis, Children


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