All issues > Volume 35(3); 1992

Original Article

J Korean Pediatr Soc. 1992;35(3):306-314. Published online March 15, 1992.

The Effec of Conditiond Media Cultued from the Thymocytes and the Splenocytes on the Regulation of Antibody Isotypes; In Vitro Immunization-III

Dong Soo DS Kim¹, Geun Woong GW Noh¹, Soon Hwan SH Oh¹

¹Department of Pediatrics, Yonsei University College of Medicine, Seoul, Korea

Abstract

We had developed a method of in-vitro immunization to increase antibody titer using several cnditioned media which were developed from mouse splenocyts and thymocytes. Using fetal calf serum, the splenocytes of the mouse were divided into two types; adherent cells and non-adherent cells. We used PHA to stimulate the adherent cells to produce PHA-stimulated adherent cell conditioned medium (ACSM-P). Non-adherent cells and thymocytes were stimulated by ACSM-P to produce ACSM-P-stimulaed non-adherent cell conditioned medium (NACSM-AP) and ACSM-P stimulaed thvmocvte conditioned medium (TmSM-AP) respectively. The highest antiody titer was observed among the methods of in-vitro immunizations. The effects of these conditioned media on producing antibody were tested in this repot. With the various combinations of these conditioned media, ivitro immunization was done, and antibody titier and isotypes of immunoglobulin were tested. ACSM-P in the presence of NACSM-AP had the effect to decrease IgM antiody response, but increase IgG reponse. On the other hands, ACSM-P in the absence of NACSM-AP showd to inhibit both IgM and IgG antibody response. TmSM-AP induced IgG antibody response, and also had anessential effect to induce IgM antibody response which was not observed in ACSM-P and NACSM-AP. All the conditioned media had their own roles to induce antibody response.l Further studies should be conducted to clartify the precise roles of these cnditioned media.

Keywords :In-Vitro Immunization