All issues > Volume 36(4); 1993

Original Article

J Korean Pediatr Soc. 1993;36(4):495-505. Published online April 15, 1993.

Effect of Cyclosporin, Indomethacin and Methylprednisolone on Puromycin-Aminonucleoside Induced Nephrosis in Rats

Hong Bae HB Kim¹, Hae Lee HL Chung¹, Cheol Woo CW Ko¹, Ja Hoon JH Ko¹, Jung Sik JS Gwak²

¹Department of Pediatrics, School of Medicine, Kyung-pook National University, Taegu, Korea
²Department of Anatomical Pathology, School of Medicine, Kyung-pook National University, Taegu, Korea

Abstract

This experimental study was conducted to evaluate the effect of indomethacin and methylprednisolone on PAN-induced nephrosis in rats. Sprague-Dawley rats weighing 150∼200gm were used and divided into controls, group Ⅰ(PAN intraperitoneally), group Ⅱ(PAN intraperitoneally, followed by indomethacin peritoneally for 12 days), group Ⅲ(PAN intraperitoneally, followed by methylprednisolone peritoneally for 12 days) and group Ⅳ(PAN intraperitoneally, followed by cyclosporin subcutaneously for 12 days). Twenty four-hour urinary protein excretion was measured on day 0, 5, 10 and 17. On the 17th day, rats were sacrificed for the determination of total serum protein, albumin and cholesterol levels. Foot process widths of glomerular epithelial cells were measured, and anionic sites of lamina rara externa were determined by using PEI as cationic probes. The following results were obtained. Twenty four-hour urinary protein excretion(mg/day) of group I was significantly increased to 455. 7±188.8 on the 5th day compared to 15.2±3.7 on day(p<0.01), and increased gradulally to 525. 6±203.5 on the 10th day, then decreased to 280.6±25.2 on the 17th day. In group Ⅲ, 24 hr srinary protein excretion on 17th day(180.7±64.5) was significantly lower than that of group Ⅰ(280.6±25.2). Total serum protein of group Ⅲ was significantly lower than that of group Ⅰ, Ⅱ, Ⅲ and Ⅳ. Foot process widths(nm) of glomerular epithelial cells in group Ⅰ, Ⅱ, Ⅲ and Ⅳ were 409.5±15.2, 387.8±49.2, 279.9±36.9 and 398.3±38.3, respectively. And the value of group Ⅲ was significantly lower than that of group Ⅰ(p<0.01). The number of anionic sites per 1㎛ length of glomerular basement membrane in Group Ⅰ, Ⅱ, Ⅲ and Ⅳ were 10.3±1.3, 10.1±1.6, 12.5±1.5 and 10.2±1.5, respectively. And the value of group Ⅲ was significantly lower than that of group Ⅰ(p<0.01). In conclusion, cyclosporin and indomethacin did not show any significant effect on PAN nephrosis in rat. However, methylprednisolone reduced the urinary protein excretion and showed significant recovery of foot process widths and number of anionic sites of glomerular basement membrane.

Keywords :Puromycin-aminonucleoside, Nephrotic syndrome, Cyclosporin, Indomethacin, Methylpredrisolone