All issues > Volume 36(8); 1993

Original Article

J Korean Pediatr Soc. 1993;36(8):1116-1123. Published online August 15, 1993.

Production and Characterization of Monoclonal Antibodies to Perchloric Acid Soluble Antigen of M. tuberculosis(TB-II)

Kyung Hyo KH Kim¹, Dong Soo DS Kim²

¹Department of Pediatrics, Ewha Woman`s University College of Medicine, Seoul, Korea
²Department of Pediatrics, Yonsei University, College of Medicine, Seoul, Korea

Abstract

Mycobacteria cause diseases which occur the world over and whoich carry a considerable burden in morbidity, mortality and social problems. A battery of monoclonal antibodies specific for mycobacterial antigens would provide a useful tool for rapid diagnosis of mycobacterial diseases. Fourtenn monoclonal antibodies to perchloric acid soluble antigne of M. tuberculosis were produced. Immunoglobulin isotypes of monoclonal antibodies were ten of immunoglobulin G2a, two of IgG3, and two of IgM. By means of Western blotting, monoclonal antibody detected the antigen of 54 kD in TB-P. In the immunofluorescence assay, the monoclonal antibody showed a positive reaction with intact M. tuberculosis bacilli, M. tuberculosis in the pulmonary tissue of tuberculous patient and M. bovis BCG.

Keywords :M. tuberculosis, Monoclonal antibodies