All issues > Volume 37(2); 1994

Original Article

J Korean Pediatr Soc. 1994;37(2):157-166. Published online February 15, 1994.

Improvement of Thoracic Pressure-Volume, Complincace and Histological Characteristics in Premature Newborn Rabbit after Treatment of Bovine Purified Natural Surfactant-Phospholipid

Chong-Woo CW Bae¹, Chang Il CI Ahn², Haruo H Maeta³, Tetsuro T Fujiwara³

¹Department of Pediatrics, College of Medicine, Kyung Hee University, Seoul, Korea
²Department of Pediatrics, College of Medicine, Korea
³Department of Pediatrics, Iwate Medical University, Morioka, Japon

Abstract

We had previously reported the purification of bovine lung natural surfactant-phospholoipd (PNS-L) and the assessment of it's surface physical properties in vitro. To observe the clinical effectiveness of PNS-L in vivo, the degree of improvement of thoracic pressure-volume, compliance and histological changes following PNS-L instillation in premature rabbits with respiratory distress syndrome (RDS) were investigated. Rabbits, delivered prematurely by cesarean section at 27days, treated with PNS-L via tracheostomy, were enrolled the study group. Two control groups were premature RDS rabbits (no treatment with PNS-L) and mature rabbits. We compared the results of thoracic pressure-volume changes during deflation phase and lung compliance changes among PNS-L treated study group and two control groups, and compared the results of aerated lung area ratio (%) on histologic samples among PNS-L treated study group and two control groups by the methods of IBSA-2000. There were significant improvements of thoracic pressure-volume during deflation phase (p<0.001), lung compliance (p<0.01, p<0.005, p<0.001) and increased aerated area histologically (p<0.005) in PNS-L treated study group compared with premature control group. It was suspected that PNS-L had contained the effective surface physical properties as a function of pulmonary surfactant. And improvement of pulmonary ventilatory functions and histological characteristics, were observed in PNS-L treated RDS model in vivo study.

Keywords :Pulmonary surfactant, Neonatal respiratory distress (RDS), Animal model of RDS, Replacement of surfactant