All issues > Volume 37(9); 1994
- Original Article
- J Korean Pediatr Soc. 1994;37(9):1226-1234. Published online September 15, 1994.
- Detection of Enterovirus in Patients with Aseptic Meningitis by Reverse Transcription and Polymerase Chain Reaction
- Souck Joong SJ Yoon1, Sung Jin SJ Hong1, Young Hyuk YH Lee1, Min Hee MH Kim1, Kyo Sun KS Kim1, Kyu Hyun KH Park2, Jae Myun JM Lee3, Won Young WY Lee3
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1Department of Pediatrics, KonKuk University College of Medicine, Seoul, Korea
2Yonsei Cancer Center
3Department of Microbiology,Yonsei University College of Medicine, Seoul, Korea
- Abstract
- The aim of this study was to determine the applicability of reverse transcription and polymerase chain reaction(RT/PCR) for routine diagnostic use and for the detection of enteroviral mentigitis, Primers were selected in the highly conserved part of the 5'-non-coding region of the enteroviral genome. Enteroviral RNA was detected by the RT/PCR in routinely collected cerebrospinal fluids(CSF) of patients with aseptic menigitis who had admitted to the dept. of Pediatrics, MinJoong Hospital, KonKuk University from May to July 1993. Enteroviral RNA was detected in one third of CSF specimen. Our results demonstrate the significance of the RT/PCR in the diagnosis of enteroviral menigitis.
Keywords :Aseptic meningitis, Reverse transcription and Polymerase Chain Reaction