All issues > Volume 37(12); 1994

Original Article

J Korean Pediatr Soc. 1994;37(12):1701-1708. Published online December 15, 1994.

House Dust Mites Sensitivity in Korean Atopic children; Correlation between Skin Reaction and Relative Concentration of Specific IgE by ELISA

Ji Hong JH Kim¹, Soo Young SY Lee¹, Byeung Ju BJ Jeung¹, Kye Earn KE Kim¹, Dong Soo DS Kim¹, Ki Young KY Lee¹

¹Department of Pediatrics, Yonsei University, College of Medicine, Seoul, Korea

Abstract

House dust mites, Dermatophagoides farinae and Dermatophagoides pteronyssius, have been identified as the main sources of house dust allergen and are considersed the most important causative allergen in the etiology of allergic respiaratory disease and allergic rhinitis. To evaluate the incidence of positive skin reactivity to house dust mites and relationship between total serum IgE levels and specific IgE antibody to house dust mites, we performed allergic skin test (scratch) in 182 children with allergic respiatory disease and atopic dermatitis. We also checked serum total IgE by PRIST method and specific IgE antibody to D. farinae and K. pteronyssinus by ELISA. Our results showed that positive skin reaction was observed in 79% of patients in D. farinae, 71% in D. pternyssinus. There was a significant correlation between serum total IgE and IgE antibody specific to D. farinae(r=0.55, p<0.001). Serum total IgE and specific IgE antibody to D. petronyssinus had no significant correlation(r=0.39, p<0.001). D. farinae and D. pteronyssinus had close correlation in the skin test(r=0.82, p<0.0001) and relative concentration of specific IgE antibody (r=0.73, p<0.0001). The agreement between skin reaction and relative concentration of specific IgE to House dust mites was 68% in D. farinae and 63% in D. pteronyssinus.

Keywords :House dust mite, Allergic skin test, Specific IgE antibody, ELISA