All issues > Volume 39(2); 1996

Original Article

J Korean Pediatr Soc. 1996;39(2):255-264. Published online February 15, 1996.

The Effect of IFN-γ and PGE2 in the Production of TNF-α by Human PBMC Stimulated with LPS

Youn Soo YS Hahn¹, Joong Gon JG Kim¹

¹Department of Pediatrics, College of Medicine, Seoul National University, Seoul,Korea

Abstract

Purpose
: The purpose of this study was to observe the effect of IFN-γ and PGE2 on TNF-α production by human peripheral mononuclear cells(PBMC) that were stimulated with LPS.
Methods
: PBMC were separated by Ficoll-Hypaque gradient centrifugation from human peripheral venous blood and were incubated for 72 hours with or without LPS, IFN-γ, PGE2, and indomethacin according to various conditions. TNF-α activity of PBMC culture supernatants was assayed by determining the cytotoxicity against L929 cells.
Results
: 1) The production of TNF-α by PBMC in response to LPS reached the highest level between 8-24 hours and returned to control level by 72 hours. 2) When IFN-γ was added together with LPS, LPS-induced TNF-α production was enhanced and prolonged, which was more remarkable when IFN-γ was added at higher concentration or earlier than LPS. 3) When PGE2 was added together with LPS, LPS-induced TNF-α production was suppressed. 4) The addition of IFN-γ reduced the suppressive effect of PGE2 on LPS-induced TNF-α production. 5) Enough amount of indomethacin enhanced production of TNF-α by LPS.
Conclusion
: IFN-γ increased and PGE2 decreased the production of TNF-α by PBMC which were stimulated with LPS. And PBMC which were pretreated with IFN-γ were resistent to the suppressive effect of PGE2.

Keywords :TNF-α, LPS, IFN-γ, PGE2