All issues > Volume 39(11); 1996

Original Article

J Korean Pediatr Soc. 1996;39(11):1576-1585. Published online November 15, 1996.

The Effect of Allopurinolon the Ischemia-reperfusion RenalInjury in Young Rats

Seung Joo SJ Lee¹

¹Department of Pediatrics, College of Medicine, Ewha Womans University, Seoul, Korea

Abstract

Objective : Oxygen free radicals has been reported to be the critical cellular mediators of experimental ischemia-reperfusion renal injury. We examined the protective effect of allopurinol (an xanthine oxidase inhibitor) on the renal damage by reperfusion in the ischemic rat kidney.
Methods
: To induce renal ischemia, the Sprague-Dawley rats(weight 70-80g) were placed in a sealed 5L jar with 8% O2-92% N2 gas mixture for 3.75 hours to the point of gasping, the first sign of asphyxia. Before returning to their metabolic cage, rats were treated with a single subcutaneous injection of allopurinol 135mg/kg or equal volume of saline as control.
Results
: Reperfusion after renal ischemia in control rats resulted in significant increase of lipid peroxidation at post-reperfusion 15 minutes, compared to postischemia values without significant change of renal function [renal microsomal malondealdehyde (MDA): 8.5¡¾0.92 vs 11.4¡¾1.72nmol/mg protein, p<0.05, renal phospholipase A2(PLA2) activity: 0.67¡¾0.04 vs 0.82¡¾0.05 pmol/mg protein/min, p<0.05, Scr: 0.60¡¾0.10 vs 0.69¡¾0.09mg/dl, p>0.05]. Allopurinol treatment before reperfusion prevented the reflow induced increase of lipid peroxidation at post-reperfusion 15 minutes compared to saline treated control rats [renal microsomal MDA: 11.4¡¾1.71 vs 5.5¡¾0.65nmol/mg protein, p<0.01), renal PLA2 activity: 0.82¡¾0.05 vs 0.32¡¾0.03 pmol/mg protein/min, p<0.01]. Ischemia-reperfusion induced renal tubular damages were prevented in allopurinol treated rats.
Conclusion
: Allopurinol treatment before reperfusion of ischemic rats reduced the renal lipid peroxidation and tubular necrosis. The role of oxygen free radicals in ischemia-reperfusion injury was indirectly confirmed.

Keywords :Renal microsomal malondealdehyde, Renal phospholipase A2 activity, Oxygen free radicals