All issues > Volume 40(8); 1997

Case Report

J Korean Pediatr Soc. 1997;40(8):1162-1168. Published online August 15, 1997.

Acute Renal Failure(ARF) Associated with Yersinia Pseudotuberculosis Infection Diagnosed by Polymerase Chain Reaction

Hyung Gyu HG Park¹, Byung Moon BM Ahn¹, Eun Ryoung ER Kim¹, Il Soo IS Kim¹, Jae Jong JJ Kim², Sung Ok SO Kang², Han Ho HH Park², Ki Jeong KJ Kim³

¹Department of pediatrics, Sung-Ae General Hospital, Seoul, Korea
²Bioneer Corp. R & D Center
³Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Korea

Abstract

The clinical significance of Yersinia pseudotuberculosis(Y. pseudotuberculosis) has recently recognized in various part of the world because it can cause a wide range of clinical problems such as mesenteric lymphadenitis, septicemia, reactive arthritis, terminal ileitis, erythema nodosum and acute renal failure. we experienced a case of acute renal failure associated with Y. pseudotuberculosis infection. We applied a nested polymerase chain reaction method for rapid diagnosis of Y. pseudotuberculosis infection. DNA was extracted from standard strains of Y. pseudotuberculosis, Y. enterocolitica, serial blood samples, urine, and mountain water by phenol-chloroform method. Using specific primers, we amplified inv and ail gene from extracted DNA samples by PCR method. The patient’ serotype was Y. pseudotuberculosis 2a and it􋺴s titer was 1:40 initially, after 2 weeks the titer increased to 1:160. Stool culture was negative. Inv gene amplification was positive in patient's urine, febrile stage blood, and mountain water. The nested polymerase chain reaction method can be used clinically for rapid diagnosis of Y. pseudotuberculosis infection. So we report here the clinical findings and PCR method of this case with brief review of the literatures.

Keywords :Yersinia pseudotuberculosis, Acute renal failure, Polymerase chain reaction