All issues > Volume 41(9); 1998

Original Article

J Korean Pediatr Soc. 1998;41(9):1198-1210. Published online September 15, 1998.

Delayed Cerebral Energy Failure After Acute Hypoxia-Ischemia in Neworn Piglet Under 48hours of Continuous Monitoring Using Near-Infrared Spectroscopy

Yun Sil Chang¹, Won Soon Park¹, Munhyang Lee¹, Ki-Soo Kim², Jung-Hwan Choi³, Son Moon Shin⁴

¹Department of Pediatrics, Samsung Medical Center, Sung Kyun Kwan University, College of Medicine, Seoul, Korea
²Department of Pediatrics, Ulsan University College of Medicine, Asan Medical Center, Seoul, Korea
³Department of Pediatrics, Seoul National University, Collge of Medicine, Seoul, Korea
⁴Department of Pediatrics, Yeungnam University College of Medicine, Taegu, Korea

Correspondence Yun Sil Chang ,Email: 1

Abstract

Purpose
: To test the hemodynamic and biochemical sequences of secondary cerebral energy failure after acute reversed hypoxic ischemic brain insult(HI) can be reproduced in newborn piglets.
Methods
: Fifteen anesthetized, ventilated newborn piglet(<3 day) were studied. Eight(HI) were induced transient HI by breathing 8% oxygen and complete occlusion of bilateral common carotid arteries for 30 minutes followed by reoxygenation and reperfusion. Seven(control) were given a sham operation. Both groups were maintained for 48 hours with intensive care and monitoring of cerbral hemodynamics and [△Cyt aa3] using near infrared spectroscopy(NIRS). Finally, brain cortex was harvested and determined activities of Na＋, K＋-ATPase, level of conjugated dienes, ATP and phosphocreatine(PCr) biochemically.
Results
: No changes took place in the 48-hour control group. In HI group, PaO2, pH, and MABP decreased, and heart rate, glucose, lactate level in blood and lactate level in CSF increased during acute HI. These variables subsequently returned to normal with time. In continuous NIRS monitoring, [△Cyt aa3] were not changed in the control group. But in HI group [△Cyt aa3] decreased significantly in acute HI and then normalized with resuscitation but gradually decreased and was significantly lower than control group at 48 hours. Cerebral Na＋, K＋- ATPase activity and ATP, PCr level of experimental group significantly decreased compared to control group. Cerebral level of conjugated dienes were not significantly different between both group.
Conclusion
: We successfully reproduced secondary cerebral energy failure after acute HI in the newborn piglet and this animal model may be useful for testing cerebroprotective strategies.

Keywords :Perinatal asphyxia, Newborn, Animal, Near infrared spectroscopy, Hypoxia, Ischemia