All issues > Volume 41(12); 1998

Original Article

J Korean Pediatr Soc. 1998;41(12):1632-1638. Published online December 15, 1998.

Effects of Cefodizime on Phagocytosis of COS-1 Ccells

Pyoung Han PH Hwang¹, Sang Yun SY Nam², Ho Keun HK Yi³, Min Ho MH Kim⁴, Kong Soo KS Kim⁴, Jung Soo JS Kim³

¹Institute for Medical Sciences, Chonbuk National University
²Department of Biology, College of Science and Technology, Chonju University
³Department of Pediatrics, School of Medicine, Chonbuk National University
⁴Department of Cardiovascular Surgery, School of M edicine, Chonbuk National University

Correspondence Pyoung Han PH Hwang ,Email: 1

Abstract

Purpose
: Cefodizime is a new third-generation cephalosporin which has a structure and immunomodutation properties similar to cefotaxime. Various studies on cefodizime have demonstrated the direct eradication of bacteria in cooperation with the host defense mechanism, particularly with phagocytosis. We evaluated the effects of cefodizime on the phagocytosis of COS-1 cells transfected with FcγRI/γγ or FcγRIIA cDNA.
Methods
: Phagocytosis was measured using the in vitro COS-1 cell modeling system according to Schreiber's method. COS-1 cells, which lack endogenoous Fcγreceptors but have phagocytic potential, were transfected with either FcγRI/γγor FcγRIIA cDNA. COS-1 cells, as target cells, were treated with antibiotics for 1 or 24 hours and incubated for 30 min with IgG coated sheep RBCs. Adhered IgG coated sheep RBCs were removed after brief exposure to hypotonic phosphate buffered saline. Phagocytosis index(PI) was calculated as the number of ingested RBCs per 100 phagocytic cells after wright-Giemsa staining.
Results
: COS-1 cells tranfected with FcγR(either FcγRI/γγ or FcγRIIA cDNA) showed the phagocytic activity against IgG coated sheep RBC, while untransfected COS-1 cells did not. After treatment with cefodizime, phagocytic activity of FcγRI/γγcDNA transfected COS-1 cells was significantly increased, while that of FcγRIIA cDNA transfected COS-1 cells did not. Marked enhancement of phagocytosis of COS-1 cells was observed after treatment with cefodizime, but was not observed with ceftriaxone or moxalactam.
Conclusion
: Cefodizime showed marked enhancement of phagocytic activity of FcγR transfected COS-1 cells. FcγRI seems to play an important role in the enhancement of phagocytosis. Further studies will be required.

Keywords :Cefodizime, In vitro COS-1 cell modeling system, Phagocytosis