All issues > Volume 42(5); 1999

Original Article

J Korean Pediatr Soc. 1999;42(5):666-671. Published online May 15, 1999.

Intralipid Infusions Effect on Nutrophil Elastase Level in Newborns

Sue Jeen SJ Min¹, Yong Woon YW Baek¹, Yong Woon YW Baek¹, Yong Woon YW Baek¹

¹Department of Pediatrics, Gospel Hospital, Kosin University, Pusan, Korea

Correspondence Sue Jeen SJ Min ,Email: 1

Abstract

Purpose
: Parenteral nutrition is given to infants who tempararily cannot take oral feeding adequately. A lipid emulsion is added to the parenteral to supply essential fatty acids. In neonatal sepsis, elastase from azuropilic granules of the neutrophils is released and rapidly bound to α1- Proteinase Inhibitor(α1-PI). The lipid emulsion has been noted to markedly inhibit chemotaxis of neutrophils, so we to measured the levels of Elastase-α1-Proteinase Inhibitor(E-α1-PI) complex to evaluate the effect of intralipid infusions on the neutrophil in newborns with sepsis
Methods
: This study evaluated 8 patients with neonatal sepsis and 12 normal newborns. We measured E-α1-PI complex levels in the serum of these patients by ELISA methods.
Results
: Before infusion with lipid solution, patients with neonatal sepsis had significantly increased levels of E-α1-PI complex in comparison with those of vaginally delivered normal newborns. E-α1-PI complex levels were significantly decreased after lipid infusions of 0.5g/kg per day, but there was no further significant decrease with higher doses of the infusate.
Conclusion
: We observed the suppression neutrophil elastase levels by lipid infusions in newborn with sepsis. These results suggest that there were no appropriate chemotatic effects of neutrophil in newborn with sepsis. Therefore, we considered whether the lipid infusion was stopped if the newborn with sepsis was infused parenteral nutrition with intralipid.

Keywords :Intralipid infusion, Elastase, Neonatal sepsis, α1-Proteinase Inhibitor