All issues > Volume 42(6); 1999

Original Article

J Korean Pediatr Soc. 1999;42(6):833-843. Published online June 15, 1999.

Molecular Cloning and Characterization of ETV1 : a New Member of the ETS Family Transcription Factor that is Implicated in Ewing's sarcoma

In-sang Is Jeon¹, David N. DN Shapiro¹

¹Department of Experimental Oncology, St. Jude Children`s Research Hospital, USA

Correspondence In-sang Is Jeon ,Email: 1

Abstract

Purpose
: Ewing's sarcoma is characterized by accompanying specific chromosome translocations. In the present study, we report ETV1, the third member of the human ETS family that is implicated in Ewing's sarcoma by reciprocal translocation (7;22)(p22;q12).
Methods
: Primarily, cDNA library made from the Ewing's sarcoma cell line accompanying the t(7;22)(p22;q12) was screened using the 5' portion of EWS(including the exon 1-7) probe on the presumption that 5' portion of the fusion gene is made of EWS and 3' is a new member of the ETS family gene. After we obtained a 3' portion of ETV1, a member of the ETS family gene, an additional 5' portion was isolated by screening a human fetal brain cDNA library, using a 326 bp NcoI fragment derived from 3' portion of coding and uncoding sequences of this gene.
Results
: We cloned the whole sequence of ETV1. Based on extensive homology, ETV1 could be subdivided into human PEA3 sub-family with ERM and E1A-F.
Conclusion
: The cloning of new oncogenes has begun to provide insight into the molecular pathogenesis of tumor formation. In this regard, the cloning of ETV1 implicated in Ewing's sarcoma might provide a clue to understand the formation of this tumor.

Keywords :Ewing's tumor, ETS gene family, ETV1