All issues > Volume 43(5); 2000

Original Article

J Korean Pediatr Soc. 2000;43(5):674-678. Published online May 15, 2000.

Change of Peripheral Blood CD5＋ B Lymphocytes in Early Neonatal Period

Ye Ho YH Lee¹, Bin B Cho¹, Department of Pediatrics, College of Medicine, Cat DC ¹, Kyung Tai KT Whang¹

¹Department of Pediatrics, College of Medicine, Catholic University of Korea
¹Department of Pediatrics, College of Medicine, Catholic University of Korea

Abstract

Purpose
: This study was aimed to investigate age-related changes of CD5＋ B lymphocytes in healthy Korean neonates.
Methods
: Sixty healthy neonatal infants were enrolled in this study; at birth(n=10), day 1(n=10), day 2(n=10), day 3(n=10), day 4(n=10) and day 5(n=10). Phenotypic analysis of CD5＋ B lymphocytes was performed on blood samples using standard flow-cytometric techniques.
Results
: The percentage of lymphocyte was significantly increased from at birth to day 5. The percentage of CD19＋ lymphocyte was decreased from at birth to day 4, but statistically not significant. The percentage of CD5＋/CD19－ lymphocyte on day 5 was significantly lower than that of at birth. The percentage of CD5－/CD19＋ lymphocyte was not changed. The percentage of CD5＋/CD19－ lymphocyte on day 1 was significantly higher than that of at birth, then it gradually decreased with aging to the at birth figure. The percentage of CD5＋/CD19＋ lymphocyte was decreased from at birth to day 5, but the changes were not significant. The percentage of CD5＋/ CD19＋ lymphocyte in CD19＋ lymphocyte was decreased from at birth to day 5, but it was not statistically significant.
Conclusion
: CD5＋ B lymphocyte counts are considerably higher than previously established adult values and the age-related changes are very important in interpretating CD5＋ B lymphocyte. These data may serve as a reference range for studies in early neonatal period.

Keywords :CD5, CD19, Newborn, Lymphocyte subset