All issues > Volume 44(4); 2001

Original Article

J Korean Pediatr Soc. 2001;44(4):397-404. Published online April 15, 2001.

The Effect of rhG-CSF and rhGM-CSF on Expression of Fcγ Receptors and β2 Integrin and Respiratory Burst Function in Human Granulocytes

Kyung Hyo KH Kim¹, Kyung Ha KH Ryu¹, Moon Kook MK Park²

¹Department of Pediatrics, College of Medicine, Ewha Woman`s University, Seoul, Korea
<sup>2</sup>Department of Microbiology, College of Medicine, Ewha Woman`s University, Seoul, Korea

Correspondence Kyung Hyo KH Kim ,Email: ewhakay@unitel.co.kr

Abstract

Purpose
: Receptors for the Fc of IgG(FcγR) and a β2 integrin molecule, CD11c/CD18 are important in clearance of microbes by granulocytes. We performed an in vitro study on the effect of recombinant human granulocyte colony-stimulating factor(rhG-CSF), or granulocyte-macrophage colony-stimulating factor(rhGM-CSF) on the expression of FcγR II, FcγR III, CD11c and CD18 and respiratory burst function of granulocytes.
Methods
: Peripheral blood was collected from six healthy adults. The isolated granulocytes were stimulated with rhG-CSF, 250μg/mL, rhGM-CSF, 100ng/mL or both of them for 1, 3, 9, 24, and 48 hours. Using flow cytometry, we compared the expression of the FcγR II, FcγR III, CD11c and CD18 of granulocytes before and after stimulation. We also the studied respiratory burst of granulocytes with chemiluminescence assay.
Results
: FcγR II and CD18 expression were not changed significantly after stimulation. FcγR III expression was decreased significantly after stimulation with rhG-CSF, rhGM-CSF, or both of them. CD11c expression was increased initially but was found to decrease significantly after 9 hours of stimulation. Granulocytes treated with rhG-CSF, rhGM-CSF, or both displayed enhanced respiratory burst. Our results suggest that exposure of granulocyte to growth factor results in granulocyte activation.
Conclusion
: We have shown that rhG-CSF and rhGM-CSF resulted in an activation of peripheral blood granulocytes immunophenotypically and functionally.

Keywords :Granulocytes, rhG-CSF, rhGM-CSF, FcγR, β2 Integrin