All issues > Volume 44(10); 2001

Original Article

J Korean Pediatr Soc. 2001;44(10):1168-1175. Published online October 15, 2001.

Serum Level of G-CSF and GM-CSF and CFU-GM Response to Hematopoietic Growth Factors in a Patient with Cyclic Neutropenia

Youn-Kyung YK Lee¹, Seok-Hun SH Kim¹, Kwang-Woo KW Kim¹, Soon S Lee², Heung-Sik HS Kim³, Dong-Seok DS Chun⁴, Kun-Soo KS Lee⁵

¹Department of Pediatrics, Sunlin Hospital, Handong University, Pohang, Korea
²Department of Pediatrics, Clinical Pathology , College of Medicine, Keimyung University, Korea
³Department of Pediatrics, College of Medicine, Keimyung University, Taegu, Korea
⁴Department of Clinical Pathology, College of Medicine, Keimyung University, Taegu, Korea
⁵Department of Pediatrics, College of Medicine, Kyungpook National University, Taegu, Korea

Abstract

Purpose
: Serum levels of G-CSF and GM-CSF were measured and CFU-GM assay using G- CSF, GM-CSF and SCF was conducted to evaluate the influence of hematopoietic growth factor on the precursor cells of cyclic neutropenia.
Methods
: A 7-year-old male with cyclic neutropenia was studied. Marrow mononuclear cells were isolated at neutrophil nadir and recovery and cultured in methylcellulose media with or without G-CSF, GM- CSF and SCF. CD34 positive cells were evaluated using flow cytometry. Serum levels of G-CSF and GM-CSF were measured by ELISA.
Results
: The Numbers of CFU-GM without growth factors were 50 at neutrophil nadir and 33 at the recovery phase in the patient and show increased colony forming capacity. CD34 positive cells were 9.32% at nadir and 14.17% at recovery. Increasement of CFU-GM with G-CSF at nadir and recovery were 46% and 118% and those with GM-CSF were 70% and 78% respectively, compared with 54.4% and 78.2% in control groups. In contrast, the presence of SCF did not enhance CFU-GM number in the patient, but in the control group, increasement with SCF was 28.9 %. There an was inverse relationship between serum G-CSF levels and peripheral neutrophil count whereas those of GM-CSF were constant.
Conclusion
: Serum G-CSF level showed inverse relationship with neutrophil counts. The response of progenitor cells to G-CSF and GM-CSF was not impaired. The presence of SCF did not enhance CFU-GM number in the patient. This result suggests that the abnormality in hematopoiesis in cyclic neutropenia may involve more immature progenitor cells responsive to SCF.

Keywords :Cyclic neutropenia, G-CSF, GM-CSF, SCF, CFU-GM, CD34