All issues > Volume 46(9); 2003

Original Article

J Korean Pediatr Soc. 2003;46(9):846-853. Published online September 15, 2003.

Serotypes and Penicillin Susceptibility of Streptococcus pneumoniae Isolated from Clinical Specimens and Healthy Carriers of Korean Children

Jin-A JA Lee¹, Nam-Hee NH Kim¹, Dong-Ho DH Kim¹, Ki-Won KW Park¹, Yun-Kyung YK Kim¹, Kyoung-Hyo KH Kim², Jin-Young JY Park³, Eun-Hwa EH Choi¹, Hoan-Jong HJ Lee¹

¹Departments of Pediatrics, Seoul National University College of Medicine, Seoul, Korea
²Departments of Pediatrics, Ewha Women's University College of Medicine, Seoul, Korea
³Departments of Pediatrics, Sungkyunkwan University College of Medicine, Kangbuk Samsung Hospital, Seoul, Korea

Correspondence Hoan-Jong HJ Lee ,Email: hoanlee@plaza.snu.ac.kr

Abstract

Purpose
: Pneumoccocus is one of the most important causes of invasive infection through the childhood period and the prevelance of antibiotics resistance of pneumococcus is increasing worldwide. A 7-valent conjugate vaccine has been developed. It is important to know the prevalence of each serotype of pneumococci in the countries where the vaccine is used to estimate the coverage rate by the vaccine.
Methods
: One hundred and twenty seven strains of clinical isolates and 72 strains from healthy carriers recovered from Korean children during the period from 1997 to 2002 were subjected to determination of serotype by Quellung reaction and penicillin susceptibility with oxacillin disc diffusion test.
Results
: Forty-three per cent of clinical isolates were obtained from children under two years of age. Thirty strains(24%) were isolated from normally sterile body fluids. The frequent serotypes were 19F, 19A, 23F, 6A, 6B and 9V. Fifty-six per cent of the clinical isolates were represented in the current 7-valent protein conjugate pneumococccal vaccine, and 84% when the cross-reactive serotypes were included. Frequent serotypes of strains isolated from one to five year-old healthy children were 19F, 14, 11A, 23F, 18C, and 19A. Seventy-one per cent of the carrier strains were included in the 7- valent vaccine. Ninety-three per cent of the clinical isolates and 86% of carrier strains were not susceptible to penicilline.
Conclusion
: Fifty-six to 84% of pneumococci recovered from Korean children are covered by the current 7-valent protein conjugate pneumococcal vaccine and the prevalence of penicillin resistance was very high.

Keywords :Streptococcus pneumoniae, Quellung reaction, Conjugate vaccine