All issues > Volume 46(11); 2003

Original Article

J Korean Pediatr Soc. 2003;46(11):1101-1106. Published online November 15, 2003.

Neuroprotective Effects of Minocycline in Rat Brain Cortical Cell Culture Induced by Hypoxia

Kyung A KA Ha¹, Bum Seok BS Yang¹, Jin Kyung JK Kim¹, Hong Tae HT Kim², Sung Jin SJ Ha³, Jong Won JW Lee³, Hai Lee HL Chung¹, Woo Taek WT Kim¹

¹Department of Pediatrics, School of Medicine, The Catholic University of Korea, Daegu, Korea
²Department of Anatomy, School of Medicine, The Catholic University of Korea, Daegu, Korea
³Department of Biochemistry, School of Medicine, The Catholic University of Korea, Daegu, Korea

Correspondence Woo Taek WT Kim ,Email: wootykim@cu.ac.kr

Abstract

Purpose
: In vivo, minocycline appears to be neuroprotective. Thus, the neuroprotective effects of minocycline were studied in a rat brain cortical cell culture induced by hypoxia.
Methods
: Cultured cells from the brains of Sprague-Dawley rats were divided into two sets of groups : normoxia groups treated with 5% CO2 and hypoxia groups treated with 1% CO2. After several days of incubation, the control groups were not treated with minocycline, while the sample groups were treated with either 1 or 10 g/mL of minocycline. The damaged cells were observed under a microscope, while apoptosis was detected using a TUNEL assay control-stained with DAPI.
Results
: Among the normoxia groups, the control and sample groups treated with 1 and 10 g/mL of minocycline were all statistically significantly different from each other. Meanwhile, among the hypoxia groups, although the control was significantly different from the sample groups, there was no statistically significant difference between the sample groups. When comparing the normoxia and hypoxia groups, there was a statistically significant difference between the control groups and sample groups treated with 1 g/mL of minocycline, yet no significant difference between the sample groups treated with 10 g/mL of minocycline.
Conclusion
: Minocycline was found to be neuroprotective in normoxia and hypoxia induced rat brain cortical cell cultures.

Keywords :Minocycline, Hypoxia, Brain, Culture