All issues > Volume 47(1); 2004

Original Article

Korean J Pediatr. 2004;47(1):81-89. Published online January 15, 2004.

Comparison of Serum Proteome Maps of Children with Kawasaki Disease

Seoung Yon SY Baek¹, Young-Mock YM Lee¹, Kwang Hoon KH Lee², Dong Soo DS Kim¹

¹Departments of Pediatrics, College of Medicine, Yonsei University, Seoul, Korea
²Departments of Dermatology, College of Medicine, Yonsei University, Seoul, Korea

Correspondence Dong Soo DS Kim ,Email: dskim6634@yumc.yonsei.ac.kr

Abstract

Purpose
: Kawasaki disease is a systemic vasculitis observed in children under five years of age and the leading cause of pediatric acquired heart disease, but its pathophysiology is still not completely understood. With proteomics, the study of quality and quantity of proteins, having been developed dramatically, we tried to find a way to evaluate the etiology and pathogenesis of Kawasaki disease by analyzing the serum proteome maps.
Methods
: We studied 10 patients admitted to the Pediatric Department of Yonsei Severance Hospital from January 2000 to July 2001. Half were diagnosed as typical Kawasaki disease and the rest as upper respiratory infection. Sera collected from the patients were frozen, melted and rehydrated for isoelectric focusing method using polyacrylamide gel and two-dimensional electrophoresis. The gel were stained by the silver method and scanned with GS-800 Calibrated Imaging Densitometer. PDQuest was used to quantify protein and draw proteome maps.
Results
: 15 protein spots of molecular weight(kDa)/isoelectric point 76.1/3.80, 75.0/4.70, 74.4/4.70, 66.3/5.77, 91.8/5.77, 125.5/5.84, 92.4/5.85, 83.8/6.09, 77.0/6.26, 76.8/6.39, 103.4/6.49, 126.5/6.40, 122.9/6.74, 35.3/6.18, 28.5/6.68 were significantly decreased in children with Kawasaki disease.
Conclusion
: Further evaluations using methods such as electrospray ionization mass spectrometry (ESI-MS), matrix-assisted laser desorption-ionization mass spectrometry(MALDI-MS) or SWISS- PROT(Swiss Institute of Bioinformatics, Geneva, Switzerland) would have to be performed to define and to understand the functions of those protein spots decreased in patients with Kawasaki disease.

Keywords :Kawasaki disease, Proteomics, Two-dimensional electrophoresis, Proteome map