All issues > Volume 47(2); 2004

Original Article

Korean J Pediatr. 2004;47(2):204-209. Published online February 15, 2004.

Effects of 7-Nitroindazole on Brain Cell Membrane Function and Energy Metabolism during Transient Global Cerebral Hypoxia-Ischemia and Reoxygenation-Reperfusion in Newborn Piglets

Chang Won CW Choi¹, Jong Hee JH Hwang¹, Yun Sil YS Chang¹, Won Soon WS Park¹

¹Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea

Correspondence Won Soon WS Park ,Email: wspark@smc.samsung.co.kr

Abstract

Purpose
: Our study was undertaken to discover whether a selective neuronal nitric oxide synthase inhibitor, 7-nitroindazole, influences brain cell membrane function and energy metabolism during and after transient global hypoxia-ischemia(HI) in newborn piglets.
Methods
: Cerebral HI was induced by temporary complete occlusion of bilateral common carotid arteries and simultaneous breathing with 8% oxygen for 30 minutes, followed by release of carotid occlusion and normoxic ventilation for one hour(reoxygenation-reperfusion, RR). 7-Nitroindazole(50 mg/kg) or vehicle was administered intraperitoneally just before the induction of HI or RR. Brain cortex was harvested for the biochemical analysis at the end of HI or RR.
Results
: The level of conjugated dienes significantly increased and the activity of Na＋, K＋-ATPase significantly decreased during HI, and they did not recover during RR. The levels of ATP and phosphocreatine(PCr) significantly decreased during HI, and recovered during RR. 7-Nitroindazole did not influence significantly the level of conjugated dienes, the activity of Na＋, K＋-ATPase, and the levels of ATP and PCr during HI and RR.
Conclusion
: 7-nitroindazole did not demonstrate a neuroprotective effect in our piglet model of transient global cerebral HI and one hour of RR.

Keywords :Hypoxia-ischemia, Brain, 7-Nitroindazole, Reperfusion injury, Metabolism, Animals, Newborn