All issues > Volume 47(6); 2004

Original Article

Korean J Pediatr. 2004;47(6):685-690. Published online June 15, 2004.

Cardioprotective Effects of Minocycline in Rat Myocardiac Cell Culture Induced by Hypoxia

Bum Seok BS Yang¹, Hye Jin HJ Park¹, Hong Tae HT Kim², Jong Won JW Lee³, Dong Suk DS Lee⁴, Un Seok US Nho⁵, Hai Lee HL Chung¹, Woo Taek WT Kim¹

¹Department of Pediatrics, School of Medicine, Catholic University of Daegu, Daegu, Korea
²Department of Anatomy, School of Medicine, Catholic University of Daegu, Daegu, Korea
³Department of Biochemistry, School of Medicine, Catholic University of Daegu, Daegu, Korea
⁴Department of Pediatrics, School of Medicine, Dong-guk University, Kyung-Ju, Korea
⁵Chilgok-Hanmaum-Yeunhap Pediatric Clinic, Daegu, Korea

Correspondence Woo Taek WT Kim ,Email: wootykim@cu.ac.kr

Abstract

Purpose
: Minocycline appears to reduce the mortality rate of patients with myocardial infarction. Therefore, the cardioprotective effects of minocycline through reduction of apoptosis were studied in a rat myocardiac cell culture induced by hypoxia.
Methods
: Cultured cells from the hearts of Sprague-Dawley rats were divided into two sets of groups : normoxia groups treated with 5% O2 and hypoxia groups treated with 1% O2. After several days of incubation, the control groups were not treated with minocycline, while the sample groups were treated with 1 g/mL of minocycline. The damaged cells were observed under a microscope, while apoptosis was detected using a Tdt-mediated dUTP nick and labeling(TUNEL) assay control- stained with 4'-6'-diamidino-2-phenylindole-2HCl(DAPI).
Results
: In normoxia, the control group showed a statistically significant difference from the sample group treated with 1 g/mL of minocycline. In addition, in hypoxia, there was also a statistically significant difference between the two groups. When comparing the normoxia and hypoxia groups to each other, there was a statistically significant difference between the control groups and sample groups.
Conclusion
: Minocycline was found to be protective for heart in normoxia and in hypoxia in the model of rat myocardiac cell culture.

Keywords :Minocycline, Hypoxia, Myocardial infarction, Myocardiac cells, Cultured