All issues > Volume 48(8); 2005

Original Article

Korean J Pediatr. 2005;48(8):881-885. Published online August 15, 2005.

Effects of Hyperosmolar Stimuli on Activation of Human Eosinophilic Leukaemia EoL-1 Cells

Byoung Chul BC Kwon¹, Eun Soo ES Kim¹, Kyung Won KW Kim¹, Tae Won TW Song¹, Myung Hyun MH Sohn², Myeong Heon MH Shin³, Kyu-Earn KE Kim²

¹Department of Pediatrics, BK 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea
²Department of Pediatrics, Institute of Allergy, BK 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea
³Department of Parasitology, Institute of Allergy, Institute of Tropical Medicine, BK 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea

Correspondence Kyu-Earn KE Kim ,Email: kekim@yumc.yonsei.ac.kr

Abstract

Purpose
: Airway dehydration and subsequent hyperosmolarity of periciliary fluid are considered critical events in exercise-induced bronchoconstriction. The aim of this study was to establish if a hyperosmolar challenge could induce activation of eosinophils.
Methods
: Human eosinophilic leukaemic cell lines, EoL-1 cells were incubated with hyperosmolar solutions for 15 minutes. Activation of EoL-1 cells was monitored by degranulation and superoxide anion production. In addition, we examined surface expression of CD69 and ICAM-1.
Results
: Hyperosmolar stimuli didn't induce superoxide anion production and degranulation. In addition, EoL-1 cells cultured with hyperosmolar medium at 930 mOsm/kg H2O resulted in no significant increment in fluorescent intensity of CD69 and ICAM-1 expression compared with results for cells incubated with isomolar medium.
Conclusion
: We found that hyperosmolar stimuli don't cause activation of EoL-1 cells, but further studies are required to determine the role of eosinophil in the mechanism of exercise-induced asthma.

Keywords :Exercise-induced asthma , Hyperosmolar , EoL-1 cells