All issues > Volume 48(8); 2005

Original Article

Korean J Pediatr. 2005;48(8):894-900. Published online August 15, 2005.

Ex vivo Expansion and Clonal Maintenance of CD34＋ Selected Cells from Cord Blood and Peripheral Blood

Soon Ki SK Kim¹, Hye Yoon HY Ghil², Sun U. SU Song², Jong Weon JW Choi³, Sang Kyu SK Park⁴

¹Department of Pediatrics , Inha University of Hospital, Inha Medical College, Incheon, Korea
²Clinical Research Center, , Inha University of Hospital, Inha Medical College, Incheon, Korea
³Department of Laboratory Medicine, Inha University of Hospital, Inha Medical College, Incheon, Korea
⁴Department of Pediatrics

Correspondence Sang Kyu SK Park ,Email: sang@uuh.ulsan.kr

Abstract

Purpose
: Because of the unavailability of marrow transplantation, umbilical cord blood (CB) is increasingly being used. We evaluated the potential of ex vivo expansion and clonality in CD34＋ cells separated from cord blood source and mobilized peripheral blood (PB) in a serum-free media.
Methods
: The CD34＋ cells, selected from CB and mobilized PB, were expanded with hematopoietic growth factors. They were then cultured for burst-forming units of erythrocytes (BFU-E), colony- forming units of granulocytes and monocytes (CFU-GM) and colony-forming units of megakaryocytes (CFU-Mk) at culture days 0, day 4, day 7, and day 14 with various growth factors.
Results
: The CB-selected CD34＋ cells showed significantly higher total cell expansion than those from the PB at day 7 (2 fold increase than PB). The CB-selected CD34＋ cells produced more BFU-E colonies than did the PB on culture at days 7 and at day 14. Also, the CB-selected CD34＋ cells produced more CFU-Mk colonies than did the PB on culture at day 4 and at day 7.
Conclusion
: The ex vivo expansion of the CB cells may be promising in producing total cellular expansion, CFU-Mk and BFU-E compared with PB for 7 to 14 days. The growth factors combination including megakaryocyte growth and development, flt3-ligand and interleukin-3 showed more expansion in the view of total cells and clonal maintenance compared with less combination.

Keywords :CD34＋ cells , Colony-forming unit of megakaryocyte , Cord blood, Mobilized peripheral blood , Ex vivo expansion