All issues > Volume 49(5); 2006

Original Article

Korean J Pediatr. 2006;49(5):545-551. Published online May 15, 2006.

Polymorphisms of the CTLA-4 promoter(-318) and exon 1(＋49) genes with atopic dermatitis in Korean children

Tae Won TW Song¹, Hea Sun HS Yang¹, Kyung Eun KE Lee¹, Kyung Won KW Kim¹, Eun Soo ES Kim¹, Myung Hyun MH Sohn¹, Kyu-Earn KE Kim, M.D¹

¹Department of Pediatrics and Institute of Allergy, BK21 Project for Medical Science, Yonsei Universi

Correspondence Myung Hyun MH Sohn ,Email: mhsohn@yumc.yonsei.ac.kr

Abstract

Purpose
: The gene-encoding cytotoxic T lymphocyte-associated antigen-4(CTLA-4) is one of the candidate genes for conferring susceptibility to atopic dermatitis(AD). The aim of the study was to investigate the association between Korean children with AD and the polymorphisms of CTLA-4 gene promoter(-318) and exon 1(＋49).
Methods
: The CTLA-4 promoter(-318 T/C) and exon 1(＋49 A/G) polymorphisms were genotyped via restriction fragment length polymorphism methods in 145 children with atopic eczema, 69 children with non-atopic eczema, and 96 healthy controls.
Results
: There was no significant difference in genotype and allele frequencies of the CTLA-4 promoter -318 T/C and exon 1 ＋49 A/G polymorphisms when the atopic eczema, non-atopic eczema, and control groups were compared. Additionally the CTLA-4 promoter -318 T/C and exon 1 ＋49 A/G polymorphisms were not shown to be associated with severity, IgE level, or eosinophil counts.
Conclusion
: Our data show that the polymorphisms within the CTLA-4 promoter(-318 T/C) and exon 1(＋49 A/G) genes are not associated with susceptibility to AD in Korean children.

Keywords :Atopic dermatitis , CTLA-4 , Polymorphism