All issues > Volume 50(12); 2007

Original Article

Korean J Pediatr. 2007;50(12):1194-1199. Published online December 15, 2007.

Quantification of endothelin-1 in human umbilical venous endothelial cell culture supernatants of small for gestational age and preeclampsia neonates

Won Kyoung WK Cho¹, So Young SY Kim¹, Chung Sik CS Chun¹

¹Department of Pediatrics, College of Medicine, Catholic University, Seoul, Korea

Correspondence So Young SY Kim ,Email: sykimped@catholic.ac.kr

Abstract

Purpose
: It was generally accepted now a days that the pathogenesis of preeclampsia, small for gestational age (SGA), intrauterine growth retardation and fetal origin of adult diseases were related with a endothelial cell dysfunction. The purpose of this study was to know the relation of such diseases by assessing the level of endothelin-1.
Methods
: SGA babies, newborns of preeclampsia and normal control mother were included in this study. Isolated endothelial cells were centrifugated and mixed with media in 37℃, 5% CO2 to obtain confluent monolayer of cultured human umbilical venous endothelial cell (HUVEC). Endothelin-1 levels were determined by Endothelin-1 colorimetric (EIA) Kits. We examined the endothelin-1 level in the HUVEC supernatants from SGA baby, and newborns from preeclampsia as well as normal mother. Also, we compared the endothelin-1 level of cultured normal HUVEC incubated with serum from cord blood of SGA, babies of preeclampsia or normal control mother.
Results
: The endothelin-1 levels in cultured HUVEC supernatants of three groups showed no significant difference but the endothelin-1 levels of cultured normal HUVEC incubated with serum from preeclampsia mother or SGA mother was significantly higher than those from newborns of control mothers (P<0.05).
Conclusion
: These findings suggest that there may be the factor which affect the endothelin-1 level in serum of cord blood from SGA and preeclampsia.

Keywords :Preeclampsia, Small for gestational age, Endothelin-1, Umbilical vein, Endothelial cel