All issues > Volume 51(1); 2008

Original Article

Korean J Pediatr. 2008;51(1):42-46. Published online January 15, 2008.

Association of apolipoprotein E polymorphisms with serum lipid profiles in obese adolescent

Jung Min JM Yoon¹, Jae Woo JW Lim¹, Eun Jung EJ Cheon¹, Kyoung Og KO Ko¹

¹Department of Pediatrics, College of Medicine, Konyang University, Daejeon, Korea.

Correspondence Eun Jung EJ Cheon ,Email: koojook@medimail.co.kr

Abstract

Purpose
: Apolipoprotein E (Apo E) plays a major role in lipoprotein metabolism and lipid transport. Many investigators have described that Apo E polymorphisms is one of the most important genetic determinants for cardiovascular disease. The purpose of this study was to evaluate the association between Apo E polymorphisms and serum lipid profiles in obese adolescent.
Methods
: We measured the serum concentrations of glucose, apolipoprotein (Apo) A1, Apo B, total cholesterol (TC), triglyceride (TG), HDL and LDL-cholesterol after overnight fasting in obese adolescent. Apo E polymorphisms were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
Results
: 86 obese adolescents participated in this study. The body mass index (BMI) of participants were excess of 95 percentile by age and sex. Male to female ratio was 1.7 and mean age of study group was 16.2±1.8 years. Mean BMI was 27.4±2.5 kg/m2. The frequency of ε2, ε3 and ε4 allele were 8.1%, 87.2% and 4.7% respectively. Study populations were classified into the following three genotypes 1) Apo E2 group (n=13, 15.1%) carrying either the ε2/ε2 or ε2/ε3 2) Apo E3 group (n=65, 75.6%) carrying the most frequent ε3/ε3 3) Apo E4 group (n=8, 9.3%) carrying either the ε3/ε4 or ε4/ε4. No differences were found among Apo E genotypes concerning age, sex, weight, height and BMI. Apo B and LDL-cholesterol concentrations were significantly higher in the Apo E4 group (P<0.05). No association were found between Apo E genotypes and glucose, Apo A1, TC, TG and HDL.
Conclusions
: We confirmed that serum concentrations Apo B and LDL-cholesterol were influenced by Apo E genotypes. Apo E polymorphisms seems to influence some alteration of lipid metabolism associated with obesity in adolescent.

Keywords :Apolipoprotein E, Polymorphism, Obesity