All issues > Volume 51(1); 2008

Original Article

Korean J Pediatr. 2008;51(1):54-61. Published online January 15, 2008.

Effects of puromycin aminonucleoside on the cytoskeletal changes of glomerular epithelial cells

Jun Ho JH Lee¹, Tae Sun TS Ha²

¹Department of Pediatrics, Pochon CHA University, College of Medicine
²Department of Pediatrics, Chungbuk National University, College of Medicine

Correspondence Tae Sun TS Ha ,Email: tsha@chungbuk.ac.kr

Abstract

of microvilli, but also separated the intercellular gaps and linear ZO-1. PAN induced oxidative stresses in time and dose dependent manners and increases of intercellular permeability in anti-oxidants inhibitable manners. High concentration of PAN induced not only actin polymerization and disorganization, but also the conglomerulation and internal dislocation of α-actinin protein. The intensities of fluorescences of ZO-1 protein were diminished and internalized by PAN in a dose-dependent manner, which were inhibited by anti anti-oxidants.
Conclusion
: PAN induced the changes of podocytes cytoskeleton and junctional barriers by way of increasing ROS in GEpC that resulted in increasing their permeability in a antioxidatn-inhibitable manner. Glomerular hyperpermeability induced by PAN mediateing through oxidative stresses is thought to take part in the mechanism of proteinuria in nephrotic syndrome.

Keywords :Puromycin aminoglycoside, Nephrotic syndrome, Proteinuria, Oxidative stresses, Anti- oxidants