All issues > Volume 51(5); 2008

Original Article

Korean J Pediatr. 2008;51(5):523-527. Published online May 15, 2008.

Transforming growth factor-β gene promoter polymorphism : its association with renal involvement in Henoch-Sch lein Purpura in childhood

Seung Ho SH Lee¹, Hwa Young HY Jee¹, Hwang Min HM Kim¹, Byung Il BI Yeh²

¹Department of Pediatrics, Wonju College of Medicine, Yonsei University, Wonju, Korea
²Department of Biochemistry, Wonju College of Medicine, Yonsei University, Wonju, Korea

Correspondence Hwang Min HM Kim ,Email: khm9120@yonsei.ac.kr

Abstract

Purpose
: Several cytokines play important roles in the inflammatory process of Henoch-Sch lein Purpura (HSP). It is likely that transforming growth factor-β (TGF-β) is involved in the pathogenesis of HSP. The purpose of this study is to investigate whether TGF-β promoter polymorphism is associated with the renal involvement of childhood HSP.
Methods
: Thirty-four patients younger than 15 years, who had been diagnosed with HSP, as well as 27 controls, were examined. Patients and controls were genotyped for TGF-β C-509T by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
Results
: The T allelic frequencies in patients and controls showed no difference (45% vs. 48.8%). No allele or genotype differences between the group of HSP group and control group were observed. The frequencies of TGF-β 509 genotypes TT, TC, and CC were no different between patients and controls (26% vs. 22%). The TT genotype of polymorphism of the TGF-β C-509T gene had no relation to the susceptibility of children to HSP and renal involvement in HSP.
Conclusion
: TGF-β T allele may not be related to the susceptibility of children to HSP. The TT genotype of polymorphism of the TGF-β C 509T gene does not appear to have an influence on renal involvement in childhood HSP.

Keywords :Henoch-Sch lein Purpura, TGF-β polymorphism, Renal involvement