All issues > Volume 34(9); 1991

Original Article

J Korean Pediatr Soc. 1991;34(9):1231-1239. Published online September 30, 1991.

Inhibitory effects of several drugs to intestinal secretory stimulation of heat-labile enterotoxin produced by enterotoxigenic E. coli.

Kyung Rae Moon¹, Sang Kee Park¹, Young Wook Chun¹, Kap Seung Kim¹, Young Bong Park¹, Young Cheon Na², Jeong Hyung Park², Hyun Kook Lee², Nam Ung Yang²

¹Department of Pediatrics, Chosun University, College of Medicine, Kwangju, Korea
²Department of Microbiology, Chosun University, College of Medicine, Kwangju, Korea

Received: March 18, 1991;  Accepted: June 14, 1991.

Abstract

For exploitation of new antidiarrheal agents to heat-labile enterotoxin (LT) produced by enterotox- igenic E. coli, we have tested several drugs by rabbit ileal loop assay and obtained some significant
results
. L-glutathione (20 mg), P-chloromercuriphenyl sulfonic acid (20 mg) and water heating extract of Prunus mume (20 mg) reduced LT-mediated intestinal fluid secretion in rabbit ileal loop assay by 92%, 87%, 92% respectively. Maleate, quinacrine HC1 and mixed antioxidant (150 mg) in usual dosage unduced diarrhea by 32%, 37%, 52% respectively. Berberine sulfate and indomethacin did not showed any drug effects. We concluded that L-glutathione and water heating extract of Prunus mume would become excellent antidiarrheal drug if these agents were enclosed with coating agent digested by intestinal enzymes.

Keywords :Enterotoxigenic E.coli;Heat-labile enterotoxin;L-gluthathione;Prunus mume