All issues > Volume 25(9); 1982

Original Article

J Korean Pediatr Soc. 1982;25(9):867-879. Published online September 30, 1982.

Allogenic bone marrow transplantation in rabbit.

Kir Young Kim, Duk Hee Kim, Young Mo Shon, Kwan Sub Chung, Hong Jin Kim, Duk Jin Yun

¹Department of Pediatrics，Yonsei University College of Medicine

Abstract

Allogenic bone marrow transplantation was done with Korean unspecified rabbits. Fresh bone marrow cell of different sex was infused to the recipient. The study group was divid- edfive groups according to immunosuppresive drugs. Each group was consited of 20 rebbits, was exposed to 1200 Rad. Group A, which were not have bone marrow transplantation, showed 100% mortality rate after 8 weeks. Group 8, which did not have immunosuppresive drug, revealed 90% mortality rate Bfter 8 weeks. GVHD was the maincause of death. (87.5%) One rabbit, which survived 1C0 days or more, had chimerism by chromosome culture. Group C, which took methotrexate, evealed 80% mortality rate after 8 weeks,also one rabbit survied 100 days or more, showed chimerism by chromosome culture. Successful marrow transplantation were 4 rabbits by chromosome culture. Group D, which received Cyclosporin A, showed 12 rabbits survived5 days or more. Am- ong them 3 rabbits survived 100 days or more. Among the cause of death, GVHD was decreased in Group D by comparing with Group B or C. We studied optimal range of radiation and found that 1200 Rad was optimal for rabbit.

Keywords :Allogenic bone marrow tranplantation, Graft versus host disease (GVHD), Chimerism, Immunosuppresion, Total body irvadiation (TBI), Methotrexate, Cyclosporin A.