All issues > Volume 10(1); 1967

Original Article

J Korean Pediatr Soc. 1967;10(1):37-42. Published online January 31, 1967.

Studies on the Measurement of Serum Bilirubin by the Eberlein's Method and a Modified Method

Sung Whan Chung¹, Young Kee Cho¹, Chang Se Kim²

¹Department of Pediatrics, Catholic Medical College
²Department of Biochemistry, Catholic Medical College

Abstract

Eberlein's simple partition method is in general use for the measurement of free bilirubin, bilirubin mono and diglucuronide which is important in the evaluation of neonatal jaundice. In this method human serum albumin is used for the preparation of standard bilirubin solution, but the human albumin is expensive and not available commercially in this country. Therefor, authors substituted human albumin with unhemolysed pooled human serum from healthy donors and compared the results with Eberlein’s method. In the author’s method the maximum absorption spectrum of UE and UB fractions is identical to that of Eberlein's method but the spectrum of LB is 430 m/z. The bilirubin concentration and the optical density show straight line correlation concordant with the Lambert-Beer’s law. The bilirubin value by the author’s method corresponds well with that of Eberlein’s method. The maternal bloods . revealed free bilirubin, bilirubin mono- and diglucuronide values of 0.564±0.266, 0,256±0.044 and 0.924±0.184； the cord bloods 1. lll±0.110, 0.362±0.049 and 0.832±0.053 mg/100 ml respectively by the author’s method.

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