All issues > Volume 17(1); 1974

Original Article

J Korean Pediatr Soc. 1974;17(1):16-27. Published online January 31, 1974.

Experimental Studies on the Diuretic Activity of the Serum of Nephrosis

Sang Woo Kim¹

¹Department of Pediatrics, College of Medicine, Seoul National University

Abstract

There were no known mechanisms concerning with the diuresis in nephrosis, spontaneous or steroid induced. Recently, several experimental studies suggest the existence of humoral factors which regulate Na metabolism in the kidney. Author made the experimental studies to find out the existence of humoral substance in the serum of nephrotic patient. Korean white male rabbits weighing 1.8 kg were gavaged with 2% NaCl selution, 100 ml 3 times hourly for hydration, and 1.0 ml of specimen (serum of nephrosis) was infused intravenously. Urine was collected through a indwelling catheter while the rabbits were placed on a holder. The following results were obtained. (1) The serum obtained from the patients of nephrosis in oliguric phase was given intravenously into the hydrated rabbits and there was no significant changes in urine volume. (2) There was a marked and prolonged increase on urine volume in hydrated rabbits after injection of serum obtained from the nephrotic patients in diuretic phase. (3) The diuretic activities of the serum in diuretic phase seems to be operated by some humoral substance and this humoral substance shows close resemblance with Rie’s “diuretic hormone in its action. (4) The serum obtained from the patient with longterm steroid medication due to nephrosis shows no changes on urine volume in hydrated rabbits except in diuretic phase, both in good responser and in resister (persistant proteinuria). Judging from the above results, some humoral substance which closely [resembles Rie’s diuretic hormone, might be existed in the serum of nephrotic patient in diuretic phase.

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