All issues > Volume 15(8); 1972

Original Article

J Korean Pediatr Soc. 1972;15(8):752-757. Published online August 31, 1972.

A Study of Factors Preparing Tetanus Toxin Sensitized Sheep Red Blood Cells

Woo-Hyun Chang¹, Seung Hoon Lee¹, Juong Kil Lee², Hahn Woong Choe²

¹Department of Microbiology, College of Medicine, Seoul National University
²Department of Pediatrics, College of Medicine, Seoul National University

Abstract

The passive hemagglutination test first described by Boyden is a most sensitive and convenient method for the measurement of humoral antibodies. Many workers attempted to determine the optimum conditions for preparing red cells sensitized with various antigens. The present paper describes experiments undertaken to find the optimum conditions for coating fresh sheep red cells with partially purified tetanus toxins for measuring minute amount of tetanus antitoxins. The optimum conditions for preparing sheep red blood cells sensitized with tetanus toxin were found as follows. 1. The optimum pH was 6.8 and 7.2. 2. The concentration of Tannic acid was 1 : 30, 000 and 1 : 40, 000. 3. The amount of toxin was 50Lf for 2.5 sheep red cell suspension and 100 Lf for 5% sheep red cell suspension. 4. The concentration of normal rabbit serum as a stabilizer was 1 : 100 dilution. 5. The sensitizing period was 10 min and/or 30 min at both 37°C and 22°C.

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