All issues > Volume 12(12); 1969

Original Article

J Korean Pediatr Soc. 1969;12(12):669-674. Published online December 31, 1969.

Detection of Tuberculin Sensitivity by Leukocyte Culture

Hyung Ro Moon¹

¹Department of Pediatrics, College of Medicine Seoul National University Seoul, Korea

Abstract

Blood specimens obtained from 15 children with positive and negative Mantoux reaction were incubated with OT, PHA-M (as a positive control) and without reagent (as a negative control) in three culture vials using leukocyte culture technique. The following results are obtained. The Mantoux-positive inactive tuberculous group showed marked lymphocytic blastogenesis including mitotic figures when cultured with OT. On the other hand, in the active tuberculous (meningitis) group blastogenesis was either absent or markedly depressed revealing no mitotic figures when cultured with OT. Blastogenesis was depressed more in the corticosteroid treated group than non corticosteroid treated group. None of the Mantoux negative control group showed blastogenesis of significance. In the Mantoux-positive inactive tuberculous group blastogenesis was depressed slightly more in the cultures with OT compared with the cultures with PHA. In the active taberculous(meningitis) group blastogensis. was markedly depressed in the cultures with PHA as well as in the cultures with OT.

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