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Original Article

Role of microRNA-498 and microRNA-410 in neonatal hypoxic ischemic encephalopathy

Eman Salah Eldeen Arafat², Hasnaa Hesham Abotaleb³, Dina Abdel Razek Midan¹, Abdel Hamid Abdo Ismail⁴, Zeinab Abouzouna¹ 

¹Pediatric Department, Faculty of Medicine, Menoufia University, Egypt., Shebin El Kom, Egypt
²Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Menoufia University, Egypt., Shebin El Kom, Egypt
³Chemistry and Entomolgy Department, Faculty of Science, Menoufia University, Egypt., Shebin El Kom, Egypt
⁴Organic Chemistry Department, Faculty of Science, Menoufia University, Egypt., Shebin El Kom, Egypt

Correspondence Zeinab Abouzouna ,Email: zeinab_sabri@yahoo.com

Received: November 1, 2024; Revised: January 29, 2025   Accepted: February 6, 2025.

Abstract

Background
Neonatal asphyxia is the primary cause of hypoxic-ischemic encephalopathy (HIE), a condition characterized by hypoxic and ischemic brain damage. A class of short noncoding RNAs known as microRNAs (miRNAs) have significant regulatory functions, can function as diagnostic and developmental indicators of diseases, and are involved in disease pathophysiology.
Purpose
To study the role of microRNA-410 and microRNA-498 in neonatal HIE as well as control and prevention of neonatal encephalopathy.
Methods
A case-control study was performed of on 30 full term neonates with proven HIE, and 30 clinically healthy full-term neonates with no evidence of HIE matched for age and sex serving as controls. The expression of microRNA-498 and microRNA-410 were measured using quantitative real-time polymerase chain reaction.
Results
Levels of miRNA-410 and miRNA-498 were higher in cases versus controls (1.56 ± 6.43 copies/ml vs 0.58 ± 0.60 copies/ml) and (55.63 ± 118.24 copies/ml vs3.74 ± 7.09 copies/ml), respectively. Of the cases, 66.7% were discharged cases and 33.3% died. Overall miRNA-410 had a sensitivity of 70%, specificity of 60%, and cut-off point of ≤0.242, whereas miRNA-498 had a sensitivity of 70%, specificity of 66.7%, and cut-off point >1.854.
Conclusion
These findings suggest that miRNA-498 and miRNA-410 can be auxiliary diagnostic and prognostic tools for neonatal HIE.

Keywords :MiRNA-498, MiRNA-410, Neonatal hypoxic-ischemic encephalopathy.