| The Role of Apoptosis in Adriamycin Induced Cardiotoxicity and Preventive Effect of L-carnitine in Rat |
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Young Mi Hong1, Seung Sook Kim2 |
1Department of Pediatrics, College of Medicine, Ewha Womans University, Seoul, Korea
2Department of Pathology, College of Medicine, Ewha Womans University, Seoul, Korea |
| 쥐에서 Adriamycin으로 유발된 심장독성에 대한 Apoptosis의 역할과 L-carnitine의 예방효과 |
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홍영미1, 김성숙2 |
1이화대학교 의과대학 소아과학교실
2이화대학교 의과대학 병리학교실 |
Correspondence:
Young Mi Hong, Email: 1
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| Abstract |
Purpose
: Adriamycin is very important in cancer chemotherapy. Unfortunately, the drug shows
cumulative dose dependent cardiotoxicity. The precise mechanism of this remains unclear. It has
been shown that adriamycin induces programmed cell death or apoptosis in many tissues. But the
role of apoptosis in adriamycin induced cardiotoxicity is uncertain.
Methods
: Sprague-Dawley rats were divided into four groups of ten animals each. All four
groups were given intraperitoneal injections for 4 weeks. The first group was given 0.9% normal
saline. The second group was injected twice a week with adriamycin(5mg/kg), the third group
with adriamycin(5mg/kg) and L-carnitine(200mg/kg).
The fourth group was injected with only L-carnitine. Cell morphology was evaluated by light and
electron-microscopy. Apoptosis was detected by labelling nicked ends of DNA fragments using
Tunnel staining kit.
Results
: There was no difference of the left ventricular internal dimension and ventricular septal
thickness among the four groups. Apoptosis was significantly increased in the adriamycin treated
group compared to the control group(P < 0.05). Addition of L-carnitine to adriamycin induced
apoptosis compared significantly to apoptosis in the adriamycin only group(P < 0.05).
Conclusion
: Apoptosis may play an important role in adriamycin induced cardiomyopathy. Lcarnitine
reduces adriamycin induced apoptosis. |
| Key Words:
Adriamycin, Cardiotoxicity, L-canitine |
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