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Clinical Utility of the Sputum Polymerase Chain Reaction Obtained by Nebulizer in the Diagnosis of Mycoplasma pneumoniae Pneumonia

Journal of the Korean Pediatric Society 1999;42(3):348-354.
Published online March 15, 1999.
Clinical Utility of the Sputum Polymerase Chain Reaction Obtained by Nebulizer in the Diagnosis of Mycoplasma pneumoniae Pneumonia
Kye Nam Yoon1, Sang Wook Park1, Eui Ho Lee1, Won Bae Lee1, Kyung Tai Whang1, Hee Jin Lee2
1Department of Pediatrics, College of Medicine, The Catholic University, Seoul, Korea
2Medical Research Institute of St. Holy Hospital, Pucheon, Korea
Mycoplasma pneumoniae 폐렴 진단시 분무기로 채취한 객담의 중합효소연쇄반응의 유용성에 관한 연구
윤계남1, 박상욱1, 이의호1, 이원배1, 황경태1, 이희진2
1가톨릭대학교 의과대학 소아과학교실
2성가병원 임상의학연구소
Abstract
Purpose
: The polymerase chain reaction(PCR) has been used for the early diagnosis of mycoplasmal infections using various clinical samples. In this study, the clinical utility of sputum PCR through a new method was studied to diagnose mycoplasma pneumonia.
Methods
: One hundred nineteen pneumonic patients, ages ranging from 15 months old to 15 years old, were examined from Aug, 1997 to Nov, 1997. We collected 99 blood samples for mycoplasma serologic tests and obtained 55 sputum specimens by nebulizer for PCR. In addition, the radiographic findings of mycoplasma pneumonia were recognized.
Results
: Mycoplasma pneumonia was diagnosed in 77 of 119 patients. The age distribution for mycoplasma pneumonia patients as follows : 11.7% in 15-24 months old, 42.8% in 2-5 years old, 36.4% in 6-10, and 9.1% in 11-15 years old. The average positive rate was 38.4% for cold agglutinin, 63.6% for antimycoplasma antibody, and 74.5% for sputum PCR. The average positive rates of antimycoplasma antibody/sputum PCR were distributed as follows : 50/100% in 15-24 months old, 60.9/59.1% in 2-5 years old, 71.9/83.3% in 6-10, and 71.4/83.3% in 11-15 years old. The radiographic findings of mycoplasma pneumonia were 62.3% in the lobar, especially the left lower lobe or segmental alveolar consolidation, 20.8% in diffuse interstitial infiltration, and 16.9% in bronchopneumonic pattern.
Conclusion
: Mycoplasma pneumonia was found in younger children and diagnosed early by sputum PCR. This method, therefore, is of considerable use.
Key Words: PCR, Sputum, Mycoplasma pneumoniae, Pneumonia


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