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Typing of Epstein-Barr Virus in Healthy Korean Children and Adults

Journal of the Korean Pediatric Society 2000;43(5):644-649.
Published online May 15, 2000.
Typing of Epstein-Barr Virus in Healthy Korean Children and Adults
Jin Han Kang1, Jae Kyun Hur1, Joeng Hyun Kim1, Ahn Hee Lee2
1Department of Pediatrics, College of Medicine, The Catholic University of Korea
2Department of Clinical Pathology, College of Medicine, The Catholic University of Korea
국내의 건강한 소아 및 성인에서 Epstein-Barr 바이러스의 감염형 분류
강진한1, 허재균1, 김종현1, 이안희2
1가톨릭대학교 의과대학 소아과학교실
2가톨릭대학교 의과대학 임상병리학교실
Abstract
Purpose
: Recently, the list of Epsterin-Barr Virus(EBV)-associated diseases has been growing. It is now known that there are two types(type 1 & 2) of EBV, but it has yet to be fully investigated as to whether type B EBV differs in any way from type A virus in terms of geographical distribution or disease association. We performed this study to find out the prevalence of the two types of EBV in EBV seropositive healthy children and adults by using one step Polymerase chain reaction(PCR).
Methods
: Fifty-four viral capsid antigen(VCA) IgG positive healthy children(5 to 15 yrs of age) and 60 healthy adults(20 to 50 yrs of age) were involved in this study. DNA extracted from saliva samples of healthy study children and adults were subjected to amplification using the modified one step general PCR, which was developed by M. Kunimoto et al for detection of EBV types. And B95-82 cells(type 1) and Jijoye cells(type 2) were used as positive controls, and K-562 cells was used as a negative control.
Results
: EBV was detected in mouth washing saliva of 8(14.8%) of 54 healthy children. Five EBV detected cases contained type 1 and the remaining three cases contained type 2. EBV was detected in mouth washing saliva of 21(35%) of 60 healthy adults. Twenty EBV detected cases contained type 1 and the remaining one was type 2. In total, EBV was detected in the saliva samples of 29 cases(25.4%), and 25(86.2%) contained type 1.
Conclusion
: The results of our study indicate that type 1 is highly dominant in Korea, and EBV excretion is more frequent in EBV positive healthy adults compared to EBV positive healthy children in Korea.
Key Words: Epstein-Barr Virus, Type, Healthy children and adults, PCR


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