| The Immunohistochemical Expression of Neuronal Nitric Oxide Synthase in Rat Hippocampus after Pentylenetetrazole-Induced Seizures |
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Doo-Kwun Kim1, Tae-Jung Jang2 |
1Department of Pediatrics, College of Medicine, Dongguk University, Kyongju, Korea
2Department of Pathology, College of Medicine, Dongguk University, Kyongju, Korea |
| Pentylenetetrazole에 의하여 유발된 경련에서 백서 해마부의 Neuronal Nitric Oxide Synthase에 대한 면역조직화학적 발현 |
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김두권1, 장태정2 |
1동국대학교 의과대학 소아과학교실
2동국대학교 의과대학 병리학교실 |
Correspondence:
Doo-Kwun Kim, Email: pedepi@dumc.or.kr
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| Abstract |
Purpose
: In order to determine the effect of neuronal nitric oxide synthase(nNOS) in seizure-related
neuronal vulnerability of the hippocampus, the expression patterns of nNOS were examined
in pentylenetetrazole(PTZ)-induced seizure groups and in PTZ seizure groups which were pretreated
with nNOS inhibitors.
Methods
: Male Sprague-Dawley rats weighing 200-300 g were used in PTZ(40 mg/kg)-induced
seizure experiments. A specific inhibitor, 50 mg/kg 7-nitroindazole(7-NI), and a non-specific inhibitor,
50 mg/kg nitro-L-arginine(L-NA) were treated 30 min before the administration of PTZ to
block nNOS. nNOS expression was evaluated by using immunohistochemical staining in the hippocampus
of each group.
Results
: The onset time of the first myoclonic jerk was markedly delayed in the 7-NI and the LNA
pretreated groups in comparison to the PTZ group. In addition, 7-NI markedly suppressed the
severity of PTZ-induced seizures. The expression of nNOS in the hippocampal CA3 area was higher
than that in CA1 area in the PTZ treated groups. In the L-NA pretreated groups, the expression
levels in the CA3 and CA1 areas were lower than those of the PTZ treated groups. Interestingly, in
the 7-NI pretreated groups, the nNOS expression levels in CA1 and CA3 areas were makedly lower
than those of PTZ and L-NA pretreated groups. There was no expression in CA2 area of all groups.
Conclusion
: These results suggest that the hippocampal neurons expressing nNOS may be vulnerable
to PTZ-induced seizures and that nNOS may play an important role in seizure-related
neuronal vulnerability. |
| Key Words:
Neuronal nitric oxide synthase, NOS inhibitor, Immunohistochemistry, Pentylenetetrazole, trazole, Seizure, Rat |
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