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Serum and Cerebrospinal Fluid(CSF) Nitric Oxide, Macrophage Inflammatory Protein-1α and Lactoferrin Levels in Aseptic Meningitis

Korean Journal of Pediatrics 2005;48(1):48-54.
Published online January 15, 2005.
Serum and Cerebrospinal Fluid(CSF) Nitric Oxide, Macrophage Inflammatory Protein-1α and Lactoferrin Levels in Aseptic Meningitis
Young Seo, Jung Yeon Shim, Jae Won Shim, Deok Su Kim, Hye Lim Jung, Moon Soo Park
Department of Pediatrics, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Korea
무균성 뇌수막염 환자의 뇌척수액과 혈청에서 Nitric Oxide, Macrophage Inflammatory Protein(MIP)-1α,Lactoferrin 값의 비교
서영, 심정연, 심재원, 김덕수, 정혜림, 박문수
성균관대학교 의과대학 강북삼성병원 소아과
Correspondence: 
Jung Yeon Shim, Email: jyssim@samsung.co.kr
Abstract
Purpose
: The pathologic mechanisms of central nervous system(CNS) injuries in human meningitis are not yet completely understood. Recent studies indicate that the host inflammatory responses are as important in brain damage as the infecting organisms and toxins. There have been some reports on the relationship of nitric oxide(NO), macrophage inflammatory protein-1α(MIP-1α), and lactoferrin in bacterial meningitis, but few reports in aseptic meningitis. Thus, we investigated the concentrations of NO, MIP-1α and lactoferrin in cerebrospinal fluid(CSF) and serum of patients with aseptic meningitis and control subjects and evaluated their relationship with other parameters of meningitis.
Methods
: CSF and blood were obtained from 25 subjects with aseptic meningitis and 15 control subjects. After centrifugation, supernatants were stored at -70℃ and we assayed the concentrations of NO, MIP-1α and lactoferrin with the ELISA method. There were no patients with neurologic sequelae after being recovered from aseptic meningitis.
Results
: Concentrations of CSF and serum NO, MIP-1α were not increased in aseptic meningitis subjects compared to control subjects. Concentration of CSF lactoferrin was significantly elevated in patients with aseptic meningitis and concentration of serum lactoferrin was significantly decreased in patients with aseptic meningitis compared with those in control subjects(P<0.05). CSF lactoferrin level was positively correlated with CSF WBC counts(rs=0.449, P=0.007), especially with neutrophil counts(rs=0.574, P<0.001) and CSF protein level(rs=0.508, P=0.002).
Conclusion
: Lactoferrin plays an important role in aseptic meningitis and may be released from neutrophils recruited from blood to the CSF through breakdown of blood-brain barrier. NO and MIP-1α may not be important factors in the pathogenesis of aseptic meningitis without neurologic sequelae.
Key Words: Meningitis aseptic, Nitric oxide, Macrophage inflammatory protein-1α, Lactoferrin


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