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Fas/FasL expression in the hippocampus of neonatal rat brains follwing hypoxic-ischemic injury

Korean Journal of Pediatrics 2006;49(2):198-202.
Published online February 15, 2006.
Fas/FasL expression in the hippocampus of neonatal rat brains follwing hypoxic-ischemic injury
Young Pyo Chang1, Myeung Ju Kim2, Young Il Lee2, Ik Je Im3, Jae Ju Cho3, Jong-Wan Kim4, Sung Moon Yeo4
1Department of Pediatrics, Institute of Life Science , College of Medicine, Dankook University, Cheon
2Department of Anatomy, College of Medicine, Dankook University, Cheonan, Korea
3Department of Pediatrics, College of Medicine, Dankook University, Cheonan, Korea
4Department of Biological Science, Dankook University, Cheonan, Korea
저산소성 허혈성 손상을 받은 신생 흰쥐 뇌 해마에서 Fas와 FasL 단백 발현
장영표1, 김명주2, 이영일2, 임익제3, 조재주3, 김종완4, 여성문4
1단국대학교 의과대학 소아과학교실, 생명과학 연구소
2단국대학교 의과대학 해부학교실
3단국대학교 의과대학 소아과학교실
4단국대학교 의과대학 첨단과학대학 생물학과
Correspondence: 
Young Pyo Chang, Email: ychang@dankook.ac.kr
Abstract
Purpose
: Fas is a cell surface receptor that transduces apoptotic death signals. Interaction of extracelluar domain of Fas with Fas ligand(FasL) triggers the apoptotic process in many diseases. We investigated the expression of Fas and FasL in the hippocampus of 7-day-old newborn rat brains following hypoxia-ischemia injury.
Methods
: The 7-days-old newborn rats were exposed to 8 percent oxygen for two hours after the ligation of right common carotid arteries. The newborn rats were killed and their brains were removed at 12, 14 and 48 hours after hypoxic-ischemic injury. The expressions of Fas and FasL of the right hippocampus were observed by western blotting and immunofluorescent staining.
Results
: Fas and FasL were strongly expressed in the right hippocampus ipsilateral to the ligation of the common carotid artery by western blotting at 12 hours following hypoxic-ischemic injury, and then slowly decreased. The immunofluorescent expressions of Fas and FasL strongly increased in the CA1 area of the right hippocampus at 12 and 24 hours following hypoxic-ischemic injury. The immunofluorescent expression of Fas decreased at 48 hours, but the expression of FasL persisted strongly at 48 hours following hypoxic-ischemic injury.
Conclusion
: The interaction of Fas with FasL on the cell surface may be involved in neuronal injury following hypoxic-ischemic injury in the developing brain.
Key Words: Fas , Fas ligand , Newborn , Brain , Hypoxia , Ischemia


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