| Effect of angiotensin II inhibition on the epithelial to
mesenchymal transition in developing rat kidney |
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Hyung Eun Yim, Kee Hwan Yoo, In Sun Bae, Young Sook Hong, Joo Won Lee |
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Department of Pediatrics, College of Medicine, Korea University, Seoul, Korea |
| 발생 중인 백서 신장에서 Angiotensin II 억제가 epithelial to mesenchymal transition에 미치는 효과 |
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임형은, 유기환, 배인순, 홍영숙, 이주원 |
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고려대학교 의과대학 소아과학교실 |
Correspondence:
Kee Hwan Yoo, Email: guroped@korea.ac.kr
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| Abstract |
Purpose
To investigate the effects of angiotensin II inhibition on the epithelial to mesenchymal transition (EMT) in the developing
kidney, we tested the expression of EMT markers and nestin in angiotensin converting enzyme (ACE) inhibitor-treated
kidneys.
Methods
Newborn rat pups were treated with enalapril (30 mg/kg/d) or a vehicle for 7 days. Immunohistochemistry for the
expression of α-smooth muscle actin (SMA), E-cadherin, vimentin, and nestin were performed. The number of positively-stained
cells was determined under 100 magnification in 10 random fields.
Results
In the enalapril-treated group, αSMA-positive cells were strongly expressed in the dilated tubular epithelial cells. The
number of αSMA-positive cells in the enalapril-treated group increased in both the renal cortex and medulla, compared to the
control group (P<0.05). The expression of E-cadherin-positive cells was dramatically reduced in the cortical and medullary tubular
epithelial cells in the enalapril-treated group (P<0.05). The number of vimentin- and nestin-positive cells in the cortex was not
different in comparisons between the two groups; however, their expression increased in the medullary tubulointerstitial cells in
the enalapril-treated group (P<0.05).
Conclusion
Our results show that ACE inhibition in the developing kidney increases the renal EMT by up-regulating αSMA and
down-regulating E-cadherin. Enalapril treatment was associated with increased expression of vimentin and nestin in the renal
medulla, suggesting that renal medullary changes during the EMT might be more prominent, and ACE inhibition might differentially
modulate the expression of EMT markers in the developing rat kidney. |
| Key Words:
Angiotensin II, Cell Transdifferentiation, Growth and Development, Kidney Diseases |
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