| Studies on the Measurement of Serum Bilirubin by the Eberlein's Method and a Modified Method
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Sung Whan Chung1, Young Kee Cho1, Chang Se Kim2 |
1Department of Pediatrics, Catholic Medical College
2Department of Biochemistry, Catholic Medical College |
| 血淸Bilirubin 測定에 對하여 |
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鄭 性 換1, 趙 永琪1, 金昌世2 |
1가톨릭醫科大學 小兒科學敎室
2가톨릭醫科大學 生化學敎室 |
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| Abstract |
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Eberlein's simple partition method is in general use for the measurement of free bilirubin, bilirubin mono and diglucuronide which is important in the evaluation of neonatal jaundice. In this method human serum albumin is used for the preparation of standard bilirubin solution, but the human albumin is expensive and not available commercially in this country. Therefor, authors substituted human albumin with unhemolysed pooled human serum from healthy donors and compared the results with Eberlein’s method. In the author’s method the maximum absorption spectrum of UE and UB fractions is identical to that of Eberlein's method but the spectrum of LB is 430 m/z. The bilirubin concentration and the optical density show straight line correlation concordant with the Lambert-Beer’s law. The bilirubin value by the author’s method corresponds well with that of Eberlein’s method. The maternal bloods . revealed free bilirubin, bilirubin mono- and diglucuronide values of 0.564±0.266, 0,256±0.044 and 0.924±0.184； the cord bloods 1. lll±0.110, 0.362±0.049 and 0.832±0.053 mg/100 ml respectively
by the author’s method.
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