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- Nephrology (Genitourinary)
- Reconsideration of urine culture for the diagnosis of acute pyelonephritis in children: a new challenging method for diagnosing acute pyelonephritis
- Jun Ho Lee, Seonkyeong Rhie
- Clin Exp Pediatr. 2019;62(12):433-437. Published online October 18, 2019
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Acute pyelonephritis (APN) should be detected and treated as soon as possible to reduce the risk of the development of acquired renal scarring. However, in the medical field, urine culture results are not available or considered when the prompt discrimination of APN is necessary and empirical treatment is started. Furthermore, urine culture cannot discriminate APN among children with febrile urinary...
- Original Article
- Validity of bag urine culture for predicting urinary tract infections in febrile infants: a paired comparison of urine collection methods
- Geun-A Kim, Ja-Wook Koo
- Clin Exp Pediatr. 2015;58(5):183-189. Published online May 22, 2015
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Purpose Catheter urine (CATH-U) and suprapubic aspiration (SPA) are reliable urine collection methods for confirming urinary tract infections (UTI) in infants. However, noninvasive and easily accessible collecting bag urine (CBU) is widely used, despite its high contamination rate. This study investigated the validity of CBU cultures for diagnosing UTIs, using CATH-U culture results as the gold standard.
Methods We retrospectively analyzed 210 infants,...
- Blood culture contamination in hospitalized pediatric patients: a single institution experience
- Hyewon Min, Cheong Soo Park, Dong Soo Kim, Ki Hwan Kim
- Clin Exp Pediatr. 2014;57(4):178-185. Published online April 30, 2014
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Purpose Blood culture is the most important tool for detecting bacteremia in children with fever. However, blood culture contamination rates range from 0.6% to 6.0% in adults; rates for young children have been considered higher than these, although data are limited, especially in Korea. This study determined the contamination rate and risk factors in pediatric patients visiting the emergency room (ER)...
- Enhanced detection and serotyping of
Streptococcus pneumoniae using multiplex polymerase chain reaction - Jong Gyun Ahn, Seong Yeol Choi, Dong Soo Kim, Ki Hwan Kim
- Clin Exp Pediatr. 2012;55(11):424-429. Published online November 23, 2012
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Purpose Methods for quick and reliable detection of
Streptococcus pneumoniae are needed for the diagnosis of pneumococcal disease and vaccine studies. This study aimed to show that sequential multiplex polymerase chain reaction (PCR) is more efficient than conventional culture in achievingS. pneumoniae -positive results.Methods Nasopharyngeal (NP) secretions were obtained from 842 pediatric patients admitted with lower respiratory infections at Severance Children's Hospital...
- Case Report
- A case report of chronic granulomatous disease presenting with aspergillus pneumonia in a 2-month old girl
- Eun Lee, Seak Hee Oh, Ji Won Kwon, Byoung Ju Kim, Jinho Yu, Chan Jeoung Park, Soo Jong Hong
- Clin Exp Pediatr. 2010;53(6):722-726. Published online June 23, 2010
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Chronic granulomatous disease (CGD) is an uncommon inherited disorder caused by mutations in any of the genes encoding subunits of the superoxide-generating phagocyte NADPH oxidase system, which is essential for killing catalase producing bacteria and fungi, such as
Aspergillus species,Staphylococcus aureus ,Serratia marcescens ,Nocardia species andBurkholderia cepacia . In case of a history of recurrent or persistent infections, immune...
- Original Article
- Effect of growth hormone on neuronal death in hippocampal slice cultures of neonatal rats exposed to oxygen-glucose deprivation
- Kyung Sik Hong, Jihui Kang, Myeung Ju Kim, Jeesuk Yu, Young Pyo Chang
- Clin Exp Pediatr. 2009;52(5):588-593. Published online May 15, 2009
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Purpose : To investigate whether growth hormone (GH) has a protective effect on neurons in hippocampal slice cultures of neonatal rats exposed to oxygen-glucose deprivation (OGD). Methods : Cultured hippocampal slices of 7-day-old rats were exposed to OGD for 60 min. Then, the slices were immediately treated with three doses of GH (5, 50, or 500 µM) in media. The... -
- Effects of electromagnetic stimulation on neurogenesis and neuronal proliferation in rat hippocampal slice culture
- Deok-Soo Kim, Eung Sang Choi, Soo Ahn Chae
- Clin Exp Pediatr. 2006;49(5):558-564. Published online May 15, 2006
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Purpose : Transcranial electromagnetic stimulation(TMS) is a noninvasive method which stimulates the central nervous system through pulsed magnetic fields without direct effect on the neurons. Although the neurobiologic mechanisms of magnetic stimulation are unknown, the effects on the brain are variable according to the diverse stimulation protocols. This study aims to observe the effect of the magnetic stimulation with two... -
- Antibiotic Sensitivity to the Major Causative Organisms of Acute Urinary Tract Infection in Children
- Yung Kwun Lee, Hee Chul Lee, Jung Mi Chun, So Young Yoon, Woo Gill Lee, Son Moon Shin
- Clin Exp Pediatr. 2005;48(7):760-765. Published online July 15, 2005
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Purpose : We studied the changes in antibiotic sensitivity to the causative organisms of urinary tract infection(UTI), in order to provide useful information on the choice of adequate drugs in the treatment of UTI. Methods : We retrospectively analyzed the major causative organisms and their antibiotic sensitivities in 69 patients diagnosed with UTI in the Department of Pediatrics, Samsung Cheil Hospital... -
- A Study of Group B Streptococcal Infection in Pregnant Women, by LIM Broth Media
- Tae Hyung Kim, Su Eun Park, Ki Hyung Kim
- Clin Exp Pediatr. 2004;47(10):1072-1075. Published online October 15, 2004
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Purpose : To evaluate the degree of Group B streptococcal colonization in Korean pregnant women of gestational age 35-37 weeks. Methods : From May 1, 2002 to April 30, 2003, the medical records of 53 pregnant(gestational age from 35 to 37 weeks) women who visited the department of Ob & Gyn, Pusan National Hospital for antenatal care, were reviewed. By use... -
- Cardioprotective Effects of Minocycline in Rat Myocardiac Cell Culture Induced by Hypoxia
- Bum Seok Yang, Hye Jin Park, Hong Tae Kim, Jong Won Lee, Dong Suk Lee, Un Seok Nho, Hai Lee Chung, Woo Taek Kim
- Clin Exp Pediatr. 2004;47(6):685-690. Published online June 15, 2004
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Purpose : Minocycline appears to reduce the mortality rate of patients with myocardial infarction. Therefore, the cardioprotective effects of minocycline through reduction of apoptosis were studied in a rat myocardiac cell culture induced by hypoxia. Methods : Cultured cells from the hearts of Sprague-Dawley rats were divided into two sets of groups : normoxia groups treated with 5% O2 and... -
- Neuroprotective Effects of Minocycline in Rat Brain Cortical Cell Culture Induced by Hypoxia
- Kyung A Ha, Bum Seok Yang, Jin Kyung Kim, Hong Tae Kim, Sung Jin Ha, Jong Won Lee, Hai Lee Chung, Woo Taek Kim
- Clin Exp Pediatr. 2003;46(11):1101-1106. Published online November 15, 2003
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Purpose : In vivo, minocycline appears to be neuroprotective. Thus, the neuroprotective effects of minocycline were studied in a rat brain cortical cell culture induced by hypoxia. Methods : Cultured cells from the brains of Sprague-Dawley rats were divided into two sets of groups : normoxia groups treated with 5% CO2 and hypoxia groups treated with 1% CO2. After several... -
- The Separate and Combined Effects of Insulin, Dexamethasone and Growth Hormone on the OB Gene Expression and Leptin Secretion from Cultured Human Visceral Adipose Tissue
- Il Tae Hwang, Kyung Hee Kim, Jin Soon Hwang, Choong Ho Shin, Sei Won Yang
- Clin Exp Pediatr. 2003;46(8):795-802. Published online August 15, 2003
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Purpose : We investigated the hormonal control of OB gene expression and leptin secretion in cultured human visceral adipose tissue. Methods : Visceral adipose tissues were cultured for up to 48 hrs in modified Eagle's medium with varying concentration of hormones : Control(no hormone), bovine insulin(100 nM), Dexamethasone(DEX, 100 nM), growth hormone(GH, 40 ng/mL), insulin＋DEX(100 nM each), insulin＋DEX＋GH(100 nM insulin... -
- Epidemiologic and Clinical Features of Pertussis in Children(2000. 3-2001. 3)
- Shin Yoo, Kyong Ouk Ahn, Eun Hye Park, Hyun Sang Cho, Chong Young Park, Hae Ran Lee
- Clin Exp Pediatr. 2002;45(5):603-608. Published online May 15, 2002
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Purpose : Pertussis is a highly communicable infectious disease in children with high mortality, especially in young infants. The incidence of pertussis in South Korea has decreased to about 10 cases a year in late '90s. Doubting previously reported incidences of pertussis, we designed this study to establish exact epidemiology and a diagnostic basis of pertussis. Methods : From Mar. 2000... -
- Case Report
- Zellweger Syndrome : First Confirmed Neonatal Case in Korea
- Sung Sin Kim, Yun Hwa An, Yun Sil Chang, Dong Kyu Jin, Won Soon Park, Moon Hang Lee, Jong Won Kim, Hye Kyoung Yoon, Bo Kyoung Kim
- Clin Exp Pediatr. 2001;44(6):694-698. Published online June 15, 2001
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We describe below a case of Zellweger syndrome case with facial dysmorphism, profound hypotonia, and hepatomegaly. He died at the age of 2 months. Zellweger syndrome is a disease marked by the absence of hepatic and renal peroxisomes. Because peroxisomes have many vital anabolic and catabolic functions within the cell, their absence results in profound cellular dysfunction. A biochemical study... -
- Original Article
- Isolation of Causative Microorganism and Antimicrobial Susceptibility Test in Impetigo Developed in the Past Four Years
- Hyun Jeong Lee, Sang Jung Lee, Seog Jun Ha, Chang Kyu Oh, Jin Wou Kim
- Clin Exp Pediatr. 2000;43(5):632-637. Published online May 15, 2000
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Purpose : Recently, Staphylococcus aureus has been reported as the principal microorganism isolated from impetigo, showing variable degrees of antimicrobial resistance. The aim of this work was to study the causative organism of impetigo the their antimicrobial susceptibility developed in impetigo in the past four years. Methods : We performed bacterial cultures and antimicrobial susceptibility tests in 73 patients with impetigo... -
- The Protective Effect of Vitamin E and Desferrioxamine on Cultured Cerebral Neurons of Neonatal Mouse Damaged by Ischemic Condition
- So-ra Lee, Yeon-kyun Oh, Seung-taek Park
- Clin Exp Pediatr. 1999;42(10):1426-1433. Published online October 15, 1999
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Purpose : Perinatal asphyxia is an important cause of neurologic morbidity. Experiments in animal models of hypoxic-ischemic brain injury demonstrate that brain damage starts during hypoxia-ischemia. In order to evaluate the ischemic condition-induced neurotoxic effect in view of oxi- dative stress, we examined the cytotoxic effect in cultured cerebral neurons of neonatal mouse. Methods : Dissociated cell cultures were prepared... -
- The Effects of Glutamate Receptor Antagonists on Cultured Cerebral Cortical Neurons of Neonatal Mouse Damaged by Oxidative Stress
- Dae-ho Choi, Yeon-kyun Oh, Seung-taek Park
- Clin Exp Pediatr. 1999;42(8):1096-1103. Published online August 15, 1999
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Purpose : To evaluate neurotoxic effects induced by oxygen-radicals, which were generated by adding xanthine oxidase(XO) and hypoxanthine(HX), and protective effects of glutamate receptor antagonist such as MK-801 and 6-cyano-7-nitroquinoxaline(CNQX). Methods : Dissociated cell cultures were prepared from cerebrum of neonatal mouse. Tissues were dissected and diced into small pieces in phosphate buffered saline and were incubated at 37℃. Isolated cells... -
- Clinical Application of a Scoring System in the Diagnosis of Group A Streptococcal Pharyngitis
- Hyeon-Bu Kim, Byung-Soo Cho, Sung-Ho Cha, Jeong-Hun Ha, Sun-Hee Kim, Gui-Sook Cho
- Clin Exp Pediatr. 1998;41(10):1365-1371. Published online October 15, 1998
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Purpose : A scoring system could be clinically useful in determining cases in which a throat culture may be omitted or in cases whether antibiotics may be initiated or not. We propose to make a clinical guideline of antibiotics administration in patients with pharyngitis. Methods : From Apr. 1996. to Mar. 1997, throat cultures were taken from 321 cases(72 cases from... -
- Optimal Number of Blood Cultures and Volume of Blood Needed to Detect Bacteremia in Children
- Jong Jae Kim, Cheol Am Kim, Sang Ho Baik, Eui Tak Oh, Hong Ja Kang, Kil Seo Kim
- Clin Exp Pediatr. 1998;41(7):917-922. Published online July 15, 1998
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Purpose : We compared pathogen recovery rates by obtaining two blood cultures instead of one blood culture containing 1ml and collecting a larger volume, 1 to 3ml. Methods : Total of 750 blood specimens from 250 patients with fever, a temperature higher than 39℃ and suspected bacteremia were obtained. Each patient had two samples of blood, A(1ml) and B(4ml), obtained at... -
- Prognosis of Bacterial Meningitis based on Cerebrospinal Fluid Culture
- Yun Ju Lee, Won Sung Lee, Kyoung Sim Kim, Ki Bok Kim
- Clin Exp Pediatr. 1998;41(5):614-621. Published online May 15, 1998
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Purpose : The present study was undertaken to assess whether the results of cerebrospinal fluid (CSF) culture are related to prognosis. Methods : Subjected to this study were 51 cases admitted to our department who had been diagnosed as bacterial meningitis. They were divided into two groups, Group Ⅰ(20) with positive CSF culture and Group Ⅱ(31) with negative outcome, then both... -
- Relationship between Antistreptolysin O Titer and Number of Colonies from Throat Swab Culture Plate in Group A Beta-hemolytic Streptococcal Carriers and Normal School Children
- Sungho Cha
- Clin Exp Pediatr. 1997;40(4):480-488. Published online April 15, 1997
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Purpose : To know the true carrier rate and the immunologic responses(antistreptolysin O :ASO) to beta-hemolytic streptococci which obtained from normal school children with or without carrying streptococci. And to study the clinical meaning of the number of organisms found in culture. Methods : One hundred ninty-eight first grade of elementary school children were included. I obtained throat swab culture from... -
- Epidemic Aseptic Meningitis in 1993 -Focused on Virus Culture-
- Ji Yeon Cho, Hyang Ju Kim, Ghee Young Jung, Jin Keun Pang, Du Bong Lee
- Clin Exp Pediatr. 1995;38(7):901-906. Published online July 15, 1995
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Purpose : Aseptic meningitis is relatively common disease caused by virus, however in most of casesthe main causes are unnoticed since virus culture and isolation is quiet difficult in our country. Authors of this thesis have conducted virus culture and isolation test to figure out cause of aseptic meningitis that was prevalent through out the country from June to July... -
- Appraisal of the Results of Throat Swab Culture Obtained from Pediatric Outpatient Clinic
- Sung-Ho Cha, Byoung-Soo Cho, Hwan-Jo Suh, Jin-Tae Suh, Seon-Ju Kim
- Clin Exp Pediatr. 1995;38(7):895-900. Published online July 15, 1995
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Purpose : We can meet a lot of patients with the upper respiratory tract infection in outpatient clinic, most of them are caused by viral in origin, which make us consider prudent prescribing antibiotics. But on the other hand, the accurate diagnosis and proper treatment of group A streptococcal upper respiratory infection should be emphasized concerning about possible development of... -
- Yersinia pseudotuberculosis Infection Confirmed by Stool Culture in Children
- Seung Nam Park, Kyeong Mi Jung, Ja Wook Koo, Churl Young Chung, Chong Rae Cho
- Clin Exp Pediatr. 1995;38(2):189-194. Published online February 15, 1995
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Purpose : The clinical significance of Y. pseudotuberculosis infection has recently recognizd in various part of the world, because it can cause a wide range of clinical problems such as mesenteric lymphadenitis, septicemia, reactive arthritis, terminal ileitis, erythema nodosum, and a cute renal failure. We have experienced 19 children with Y. pseudotuberculosis infection confirmed by stool culture. Our aim in... -
- Posterior Nasal Swab Culture in Normal Newborns by Feeding Method.
- Young Youn Choi, Tai Ju Hwang, Jae Sook Ma, Kwang Ok Lee, Byung Hee Kim, Young Soo Chung, En Kyeong Bom, Too Yong Yoo
- Clin Exp Pediatr. 1990;33(4):472-480. Published online April 30, 1990
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To evaluate the difference of normal flora between breast and formula-fed newborns, we performed quantitative posterior nasal swab culture in 21 breast-and 44 formula-fed newborns. Positive culture rates of total aerobes and total anaerobes were 100% and 98%, respecitively. Among the aerobes, positive culture rate was 100% in group B streptococcus, 98% in gram negative bacteria, 86% in staphylococcus aureus, 82% in staphylococcus epidermidis... -
- Clinical Evaluation of Gastric Juice Culture of Newborn.
- Byoung Hoon Lee, Back Hee Lee, Soon Wha Kim, Keum Chan Sohn
- Clin Exp Pediatr. 1989;32(3):342-348. Published online March 31, 1989
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A clinical evaluation of gastric juice culture was done on 545 newborn at the N.M.C. Jan. 1st 1985 and Dec. 31, 1986. The results were as follows; 1) There were no significant statistic difference of biπh weight, gestational age, maternal age, labor duration, delivery type and duration of rupture of membrane between gastric juice culture positive and negative groups. 2) Apgar score after 1 minute was... -
- A Clinical Study of Meconium Stained Baby and Culture of the Tracheal and Gastric Meconium.
- Dong Ryong Lee, Sung Ho Bae, Yeon Kyun Oh, San Ho Kim
- Clin Exp Pediatr. 1988;31(12):1580-1587. Published online December 31, 1988
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Among the total of 3,102 newborns delivered at Won Kwang University Hospital from September 1984 to December 1986, a clinical study was performed of meconium stained baby and culture of the tracheal and gastric meconium. The results were as follows: 1) The incidence of meconium staining was 8.8% (272 cases) and the positive rate of meconium culture was 22.8% (62 cases). 2) The cultures of tracheal and gastric... -
- Clinical Analysis on Organisms Isolated from Blood Culture.
- Dong Min Kang, Jong Seo Lee, Hong Dae Cha, Tae Chan Kwon, Chin Moo Kang
- Clin Exp Pediatr. 1988;31(1):40-47. Published online January 31, 1988
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This is the report of clinical analysis on 197 organisms isolated from blood culture for 8,385 patients which were admitted to the Pediatric department of Dong San Hospital, Keimyung University, during the priod of 3 years from June 1982 to May 1985. The results were as follows Out of 197 cases, 81 (41.4%) were above 5 years of age. Out of 197 cases, 117 (59.4%)... -
- Bone Marrow Cell Culture(GM-CFU) in Anaplastic Anemia of Children.
- Jae Sun Jung, Chang Yee Hong
- Clin Exp Pediatr. 1985;28(9):888-898. Published online September 30, 1985
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Bone marrow cell cultures (GM-CFU) were performed in the 15 cases of children with aplastic anemia and the correlation with the clinical course were investigated. They have been treated with androgen with or without low dose prednisone and supportive care. Bone marrow cell cultures from 8 children without any evidence of aplastic anemia were performed as a control. 1)In control... -
- Culture Study in Neonatal infection.
- Ja Ye Kim, Seung Joo Lee, Keun Lee
- Clin Exp Pediatr. 1984;27(5):433-438. Published online May 31, 1984
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Bacterial infection is a frequent and important cause of morbidity and mortality in the neonatal period. Knowing the changing pattern of neonatal bacterial infections is important and recently the group B streptococcus has been vieing with Coliforms for preeminence. This study was undertaken to observe the pattern of bacteria in the neonatal infections and for detection of group B streptococcus.... -
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