| The Effect of Testosterone on the Response of Grow Hormone Secretion in Cultured Rat Pituitary Cells |
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Ho Seong Kim1, Duk Hi Kim2, Deok Bae Park3 |
1Department of Pediatrics, Ewha University, College of Medicine, Seoul, Korea
2Department of Pediatrics, College of Medicine, Yonsie University, Seoul, Korea
3Department of Endocrine Laboratory, College of Medicine, Yonsie University, Seoul, Korea |
| 흰쥐 뇌하수체전엽 배양세포에서 테스토스테론이 성장호르몬 분비에 미치는 영향 |
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김호성1, 김덕희2, 박덕배3 |
1이화여자대학교 의과대학 소아과학교실
2연세대학교 의과대학 소아과학교실
3연세대학교 의과대학 중앙연구실 |
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| Abstract |
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The effects of testosterone on the pituitary growth hormone(GH) response directly and to hypothalamic growth hormone-releasing hormone(GHRH) were evaluated in vitro using a male pituitary cell monolayer culture system. Wistar male rats were gonadectomized at 22 days of age, and 21 days later their anterior pituitaries were removed and trypsinized for cell dispersion. Testosterone 0.01, 1.0, 10.0nM was added to the medium for 1 day and GH amounts in media were measured. In another experiment, testosterone 0, 0.1, 1.0, 5.0, 10.0nM was added to the medioum for 3 days, and subsequently 5nM GHRH was added for 1 day, thereafter GH amounts in media were measured. The results were as follows :
1) The increase of GH response after testosterone administration to the cultured rat pituitary cell was not significant.
2) The rat pituitary cell response to GHRH was augmented after pretreatment with testosterone.
These results are suggested that testosterone has no direct effect on GH secertion, but by incereasing the pituitary cell response to GHRH, contributes to the regulation of GH secretion in vitro. |
| Key Words:
Testosterone, Rat anterior pituitary, Growth hormone-releasing hormone |
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