| Improvement of Thoracic Pressure-Volume, Complincace and Histological Characteristics in Premature Newborn Rabbit after Treatment of Bovine Purified Natural Surfactant-Phospholipid |
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Chong-Woo Bae1, Chang Il Ahn2, Haruo Maeta3, Tetsuro Fujiwara3 |
1Department of Pediatrics, College of Medicine, Kyung Hee University, Seoul, Korea
2Department of Pediatrics, College of Medicine, Korea
3Department of Pediatrics, Iwate Medical University, Morioka, Japon |
| 토끼 태아 RDS 모델에서 Bovine Purified Natural Surfactant 보충요법 후 폐기능 및 조직학적 개선에 관한 연구 |
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배종우1, 안창일2, 前多治雄3, 藤原哲郞3 |
1경희대학교 의과대학 소아과학교실
2경희대학교 의과대학 소아과학교실
3日本岩手醫科大學 小兒科 |
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| Abstract |
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We had previously reported the purification of bovine lung natural surfactant-phospholoipd (PNS-L) and the assessment of it's surface physical properties in vitro.
To observe the clinical effectiveness of PNS-L in vivo, the degree of improvement of thoracic pressure-volume, compliance and histological changes following PNS-L instillation in premature rabbits with respiratory distress syndrome (RDS) were investigated. Rabbits, delivered prematurely by cesarean section at 27days, treated with PNS-L via tracheostomy, were enrolled the study group. Two control groups were premature RDS rabbits (no treatment with PNS-L) and mature rabbits.
We compared the results of thoracic pressure-volume changes during deflation phase and lung compliance changes among PNS-L treated study group and two control groups, and compared the results of aerated lung area ratio (%) on histologic samples among PNS-L treated study group and two control groups by the methods of IBSA-2000.
There were significant improvements of thoracic pressure-volume during deflation phase (p<0.001), lung compliance (p<0.01, p<0.005, p<0.001) and increased aerated area histologically (p<0.005) in PNS-L treated study group compared with premature control group.
It was suspected that PNS-L had contained the effective surface physical properties as a function of pulmonary surfactant. And improvement of pulmonary ventilatory functions and histological characteristics, were observed in PNS-L treated RDS model in vivo study. |
| Key Words:
Pulmonary surfactant, Neonatal respiratory distress (RDS), Animal model of RDS, Replacement of surfactant |
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