| The Effect of IFN-γ and PGE2 in the Production of TNF-α by Human PBMC Stimulated with LPS |
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Youn Soo Hahn, Joong Gon Kim |
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Department of Pediatrics, College of Medicine, Seoul National University, Seoul,Korea |
| Lipopolysaccharide에 의해 자극된 사람의 말초 혈액 단핵구의 Tumor Necrosis Factor-α 생성에 미치는 Interferon-γ와 Prostaglandin E2의 영향 |
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한윤수, 김중곤 |
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서울대학교 의과대학 소아과학교실 |
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| Abstract |
Purpose
: The purpose of this study was to observe the effect of IFN-γ and PGE2 on TNF-α
production by human peripheral mononuclear cells(PBMC) that were stimulated with LPS.
Methods
: PBMC were separated by Ficoll-Hypaque gradient centrifugation from human
peripheral venous blood and were incubated for 72 hours with or without LPS, IFN-γ, PGE2,
and indomethacin according to various conditions. TNF-α activity of PBMC culture supernatants
was assayed by determining the cytotoxicity against L929 cells.
Results
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1) The production of TNF-α by PBMC in response to LPS reached the highest level between
8-24 hours and returned to control level by 72 hours.
2) When IFN-γ was added together with LPS, LPS-induced TNF-α production was enhanced
and prolonged, which was more remarkable when IFN-γ was added at higher concentration or
earlier than LPS.
3) When PGE2 was added together with LPS, LPS-induced TNF-α production was
suppressed.
4) The addition of IFN-γ reduced the suppressive effect of PGE2 on LPS-induced TNF-α
production.
5) Enough amount of indomethacin enhanced production of TNF-α by LPS.
Conclusion
: IFN-γ increased and PGE2 decreased the production of TNF-α by PBMC which
were stimulated with LPS. And PBMC which were pretreated with IFN-γ were resistent to the
suppressive effect of PGE2. |
| Key Words:
TNF-α, LPS, IFN-γ, PGE2 |
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