| Diagnosis of Bordetella Pertussis Infections by Polymerase Chain Reaction |
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Mi Ran Kim2, Hee Jung Kang3, Hoan Jong Lee1 |
1Department of Pediatrics, Seoul National University College of Medicine, Seoul, Korea
2Department of Pediatrics, College of Medicine, Hallym University, Seoul, Korea
3Department of Clinical Pathology, College of Medicine, Hallym University, Seoul, Korea |
| 중합효소연쇄반응을 이용한 Bordetella pertussis 감염의 진단 |
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김미란2, 강희정3, 이환종1 |
1서울대학교 의과대학 소아과학교실
2한림대학교 의과대학 소아과학교실
3한림대학교 의과대학 임상병리학교실 |
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| Abstract |
Purpose
: Pertussis, a respiratory tract infection caused by Bordetella pertussis, is an important
cause of morbidity in children. But diagnosis of pertussis is often delayed because of late
development of typical symptoms and difficulties in culture. There has been no bacteriologically
confirmed case of B. pertussis infection in Korea. Lower respiratoy tract may be involved in
pertussis. We performed the polymerase chain reaction(PCR) assay for B. pertussis from children
with lower respiratory tract infections.
Methods
: One hundred eighty nine nasopharyngeal aspirates were collected from children with
lower respiratory tract infections during the period from November 1990 to February 1995. Three
24-mer primers derived from DNA sequences upstream of the structural genes for the porin
proteins of Bordetella: P1 is shared by all three species and P2 is specific for B. pertussis and
P3 is specific for B. parapertussis and B. bronchiseptica. Amplifications resulted in 159-bp PCR
products specific for B. pertussis and 121-bp PCR products specific for B. parapertussis and B.
bronchiseptica. A confirmatory cleavage of 159-bp PCR products of B. pertussis by Hae III
revealed two bands of 22 and 137 bp.
Results
: B. pertussis specific PCR products were visualized in 6 patients during 1991 and 5
of these had received appropriate doses of the combined DPT vaccine. They had had cough over
2 weeks in all and high fever in 4. They had been diagnosed as viral pneumonia in 5 and
bronchiolitis in 1, but viral cultures for respiratory syncytial virus, parainfluenza virus, influenza
virus, adenovirus were negative. There was no PCR product compatible with B. parapertussis or
B. bronchiseptica.
Conclusion
: PCR assay is effective in diagnosis of B. pertussis infections. We suggest that
there was an epidemic of pertussis in 1991 despite high rate of pertussis vaccine coverage in
Korea. |
| Key Words:
Pertussis, Polymerase chain reaction, Lower respiratory tract infections |
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