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The Effect of Allopurinolon the Ischemia-reperfusion RenalInjury in Young Rats

Journal of the Korean Pediatric Society 1996;39(11):1576-1585.
Published online November 15, 1996.
The Effect of Allopurinolon the Ischemia-reperfusion RenalInjury in Young Rats
Seung Joo Lee
Department of Pediatrics, College of Medicine, Ewha Womans University, Seoul, Korea
Allopurinol이 어린 흰쥐의 허혈 재관류성 신손상에 미치는 영향
이승주
이화여자대학교 의과대학 소아과학교실
Abstract
Objective : Oxygen free radicals has been reported to be the critical cellular mediators of experimental ischemia-reperfusion renal injury. We examined the protective effect of allopurinol (an xanthine oxidase inhibitor) on the renal damage by reperfusion in the ischemic rat kidney.
Methods
: To induce renal ischemia, the Sprague-Dawley rats(weight 70-80g) were placed in a sealed 5L jar with 8% O2-92% N2 gas mixture for 3.75 hours to the point of gasping, the first sign of asphyxia. Before returning to their metabolic cage, rats were treated with a single subcutaneous injection of allopurinol 135mg/kg or equal volume of saline as control.
Results
: Reperfusion after renal ischemia in control rats resulted in significant increase of lipid peroxidation at post-reperfusion 15 minutes, compared to postischemia values without significant change of renal function [renal microsomal malondealdehyde (MDA): 8.5¡¾0.92 vs 11.4¡¾1.72nmol/mg protein, p<0.05, renal phospholipase A2(PLA2) activity: 0.67¡¾0.04 vs 0.82¡¾0.05 pmol/mg protein/min, p<0.05, Scr: 0.60¡¾0.10 vs 0.69¡¾0.09mg/dl, p>0.05]. Allopurinol treatment before reperfusion prevented the reflow induced increase of lipid peroxidation at post-reperfusion 15 minutes compared to saline treated control rats [renal microsomal MDA: 11.4¡¾1.71 vs 5.5¡¾0.65nmol/mg protein, p<0.01), renal PLA2 activity: 0.82¡¾0.05 vs 0.32¡¾0.03 pmol/mg protein/min, p<0.01]. Ischemia-reperfusion induced renal tubular damages were prevented in allopurinol treated rats.
Conclusion
: Allopurinol treatment before reperfusion of ischemic rats reduced the renal lipid peroxidation and tubular necrosis. The role of oxygen free radicals in ischemia-reperfusion injury was indirectly confirmed.
Key Words: Renal microsomal malondealdehyde, Renal phospholipase A2 activity, Oxygen free radicals


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