| The Effect of Allopurinolon the Ischemia-reperfusion RenalInjury in Young Rats |
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Seung Joo Lee |
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Department of Pediatrics, College of Medicine, Ewha Womans University, Seoul, Korea |
| Allopurinol이 어린 흰쥐의 허혈 재관류성 신손상에 미치는 영향 |
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이승주 |
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이화여자대학교 의과대학 소아과학교실 |
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| Abstract |
Objective : Oxygen free radicals has been reported to be the critical cellular mediators of
experimental ischemia-reperfusion renal injury. We examined the protective effect of allopurinol
(an xanthine oxidase inhibitor) on the renal damage by reperfusion in the ischemic rat kidney.
Methods
: To induce renal ischemia, the Sprague-Dawley rats(weight 70-80g) were placed in a
sealed 5L jar with 8% O2-92% N2 gas mixture for 3.75 hours to the point of gasping, the first
sign of asphyxia. Before returning to their metabolic cage, rats were treated with a single
subcutaneous injection of allopurinol 135mg/kg or equal volume of saline as control.
Results
: Reperfusion after renal ischemia in control rats resulted in significant increase of
lipid peroxidation at post-reperfusion 15 minutes, compared to postischemia values without
significant change of renal function [renal microsomal malondealdehyde (MDA): 8.5¡¾0.92 vs
11.4¡¾1.72nmol/mg protein, p<0.05, renal phospholipase A2(PLA2) activity: 0.67¡¾0.04 vs
0.82¡¾0.05 pmol/mg protein/min, p<0.05, Scr: 0.60¡¾0.10 vs 0.69¡¾0.09mg/dl, p>0.05]. Allopurinol
treatment before reperfusion prevented the reflow induced increase of lipid peroxidation at
post-reperfusion 15 minutes compared to saline treated control rats [renal microsomal MDA:
11.4¡¾1.71 vs 5.5¡¾0.65nmol/mg protein, p<0.01), renal PLA2 activity: 0.82¡¾0.05 vs 0.32¡¾0.03
pmol/mg protein/min, p<0.01]. Ischemia-reperfusion induced renal tubular damages were
prevented in allopurinol treated rats.
Conclusion
: Allopurinol treatment before reperfusion of ischemic rats reduced the renal lipid
peroxidation and tubular necrosis. The role of oxygen free radicals in ischemia-reperfusion
injury was indirectly confirmed. |
| Key Words:
Renal microsomal malondealdehyde, Renal phospholipase A2 activity, Oxygen free radicals |
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